PXD009443

PXD009443 is an original dataset announced via ProteomeXchange.

Title	Temporal profiling of redox-dependent heterogeneity in single cells
Description	Cellular redox status affects diverse cellular functions, including proliferation, protein homeostasis, and aging. Thus, individual differences in redox status can give rise to distinct sub-populations even among cells with identical genetic backgrounds. Here, we have created a novel methodology to track redox status at single cell resolution using the redox-sensitive probe roGFP. Our method allows identification and sorting of sub-populations with different oxidation levels in either the cytosol, mitochondria or peroxisomes. Using this approach we defined redox-dependent heterogeneity of yeast cells, and characterized growth, as well as proteomic and transcriptomic profiles of subpopulations of cells that differ in their redox status, but are similar in age. We report that, starting in late logarithmic growth, cells of the same age have a bi-modal distribution of oxidation status. A comparative proteomic analysis between these populations identified three key proteins, Hsp30, Dhh1, and Pnc1, which affect basal oxidation levels and may serve as first line of defense proteins in redox homeostasis.
HostingRepository	PRIDE
AnnounceDate	2018-06-18
AnnouncementXML	Submission_2018-06-18_05:24:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meytal Radzinski
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-09 06:15:15	ID requested
1	2018-06-18 00:54:41	announced
⏵ 2	2018-06-18 05:24:34	announced	Updated publication reference for PubMed record(s): 29869985.

Radzinski M, Fassler R, Yogev O, Breuer W, Shai N, Gutin J, Ilyas S, Geffen Y, Tsytkin-Kirschenzweig S, Nahmias Y, Ravid T, Friedman N, Schuldiner M, Reichmann D, Temporal profiling of redox-dependent heterogeneity in single cells. Elife, 7():(2018) [pubmed]

curator keyword: Biological

submitter keyword: redox, yeast

Dr. Dana Reichmann
contact affiliation	Dept. of Biological Chemistry The Alexander Silberman Institute of Life Science The Hebrew University of Jerusalem
contact email	danare@mail.huji.ac.il
lab head
Meytal Radzinski
contact affiliation	Hebrew University of Jerusalem
contact email	meytal.radzinski@mail.huji.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009443

PRIDE project URI

• PRIDE
  1. PXD009443
     1. Label: PRIDE project
     2. Name: Temporal profiling of redox-dependent heterogeneity in single cells