PXD009437

PXD009437 is an original dataset announced via ProteomeXchange.

Title	Human intestine LC-MSMS
Description	Aflatoxin M1 (AFM1) is a common mycotoxin in dairy milk and it is typically concurrently present with other mycotoxins that may represent a threat for food safety. However, knowledge on how AFM1, alone or in combination with other mycotoxins may affect human intestinal epithelial integrity remain to be established. We employed transcriptome and proteome analysis integrated with biological validation to reveal the molecular basis underlining the effect AFM1 and/or ochratoxin A (OTA) exposure on intestinal epithelial integrity of differentiated Caco-2 cells. Exposure to 4 μg/ml of OTA was found to disrupt human gut epithelial integrity, whereas 4 μg/ml of AFM1 did not. Integrated transcriptome and proteome analysis of AFM1 and OTA, alone or in combination, indicate synergistic effect of the two mycotoxins in disrupting intestinal integrity. This effect was mechanistically linked to a broad ranges of pathways related to intestinal integrity enriched by down-regulated genes and proteins, associated to focal adhesion, adherens junction, and gap junction pathways. Furthermore, the cross–omics analysis of mixed AFM1 and OTA compared with OTA alone suggest that kinases family members, including MLCK, MAPKs, and PKC are the potential key regulators on modulating intestinal epithelial integrity. These findings provide novel insight into the synergistic detrimental role of multiple mycotoxins in disrupting intestinal integrity, and, therefore, identify potential target to improve milk safety related to human health.
HostingRepository	PRIDE
AnnounceDate	2018-08-08
AnnouncementXML	Submission_2018-08-08_09:20:36.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009437
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yanan Gao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-09 02:14:11	ID requested
⏵ 1	2018-08-08 09:20:38	announced

Gao Y, Li S, Bao X, Luo C, Yang H, Wang J, Zhao S, Zheng N, Transcriptional and Proteomic Analysis Revealed a Synergistic Effect of Aflatoxin M1 and Ochratoxin A Mycotoxins on the Intestinal Epithelial Integrity of Differentiated Human Caco-2 Cells. J Proteome Res, 17(9):3128-3142(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, intestinal epithelial cells, LC-MSMS

Jiaqi Wang
contact affiliation	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China
contact email	jiaqiwang@vip.163.com
lab head
Yanan Gao
contact affiliation	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
contact email	gyn758521@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009437
     1. Label: PRIDE project
     2. Name: Human intestine LC-MSMS