PXD009433

PXD009433 is an original dataset announced via ProteomeXchange.

Title	MET-dependent modulation of cellular responses to ionizing radiation by quantitative phosphoproteomics
Description	Increasing evidence suggests that inhibiting growth factor receptor tyrosine kinases (RTKs) signaling may modulate cellular responses to DNA-damaging agents widely used in cancer treatment. In that respect, the MET RTK is deregulated in abundance and/or activity in a variety of human tumors. Using two proteomic techniques, we explored how the MET inhibitor tepotinib modulates global cellular phosphorylation response to ionizing radiation (IR). Following an immunoaffinity-based phosphoproteomic discovery survey we selected candidate phosphorylation sites for extensive characterization by targeted proteomics focusing on phosphosites that consist of the SQ motif recognized by the PIKK-related kinases ATM, ATR and PRKDC. Several substrates of the DNA damage response (DDR) were confirmed to be modulated by sequential MET inhibition and IR, or MET inhibition alone.
HostingRepository	PRIDE
AnnounceDate	2020-05-15
AnnouncementXML	Submission_2020-05-15_00:06:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ariel Bensimon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q TRAP; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-09 00:23:27	ID requested
⏵ 1	2020-05-15 00:06:06	announced

Bensimon A, Koch JP, Francica P, Roth SM, Riedo R, Gl, ü, ck AA, Orlando E, Blaukat A, Aebersold DM, Zimmer Y, Aebersold R, Medov, á M, Deciphering MET-dependent modulation of global cellular responses to DNA damage by quantitative phosphoproteomics. Mol Oncol, 14(6):1185-1206(2020) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: phosphoproteomics, selected reaction monitoring (SRM), targeted proteomics, immunoaffinity

Ruedi Aebersold
contact affiliation	Institute of Molecular Systems Biology Department of Biology ETH Zurich
contact email	aebersold@imsb.biol.ethz.ch
lab head
Ariel Bensimon
contact affiliation	ETH Zurich
contact email	bensimon@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD009433

PRIDE project URI

• PRIDE
  1. PXD009433
     1. Label: PRIDE project
     2. Name: MET-dependent modulation of cellular responses to ionizing radiation by quantitative phosphoproteomics