PXD009426

PXD009426 is an original dataset announced via ProteomeXchange.

Title	Sample preparation for proteomics
Description	Urea-containing buffer solutions are generally used in proteomic studies to aid protein denaturation and solubilisation during cell and tissue lysis. It is well-known, however, that urea can lead to the carbamylation of peptides and proteins and; subsequently, incomplete digestion of proteins. In our work, we sought for ways to retain urea in the digestion protocol to reduce sample handling, while minimizing urea-related drawbacks.
HostingRepository	PRIDE
AnnounceDate	2018-06-06
AnnouncementXML	Submission_2018-06-06_00:02:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lazaro Hiram Betancourt Nunez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	carbamoylated residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-06 03:41:07	ID requested
⏵ 1	2018-06-06 00:02:07	announced

Betancourt LH, Sanchez A, Pla I, Kuras M, Zhou Q, Andersson R, Marko-Varga G, C. J Proteome Res, 17(7):2556-2561(2018) [pubmed]

curator keyword: Technical

submitter keyword: urea, LC-MS/MS, Lys-C/trypsin

Gyorgy Marko-Varga
contact affiliation	Div. Clinical Protein Science & Imaging Dept. of Clinical Sciences (Lund) and Dept. of Biomedical Engineering Lund University
contact email	gyorgy.marko-varga@bme.lth.se
lab head
Lazaro Hiram Betancourt Nunez
contact affiliation	Lund University
contact email	lazaro.betancourt@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009426

PRIDE project URI

• PRIDE
  1. PXD009426
     1. Label: PRIDE project
     2. Name: Sample preparation for proteomics