PXD009418

PXD009418 is an original dataset announced via ProteomeXchange.

Title	Analysis of the preserved amino acid bias in peptide profiles of Iron Age teeth from a tropical environment enabling sexing of individuals using amelogen in MRM
Description	We describe the first dental proteomic profiles of Iron Age individuals (c2000-1000 years B.P), collected from the site of Long Long Rak rock shelter (LLR) in northwest Thailand. A bias toward the preservation of small, acidic and hydrophobic amino acids is observed. It is evident that the 212 proteins identified (2 peptide, FDR <1%) comprise a palimpsest of alterations that occurred both ante-mortem and post-mortem. Conservation of particular amino acids has contributed to the identification of amelogenin peptides. A novel MRM method for sexing individuals using the amelogenin protein is described, with four teeth indicating male origin. Stable isotope analysis using carbon and oxygen isotopes highlights the strongly C3 based (~80%) diet of the Long Long Rak cemetery people, which probably comprised rice combined with protein from freshwater fish among other food items. The combination of pathway and isotopic analysis adds weight to the relatively simple C3 based diets having contributed to the enrichment of pathways associated with metabolic conditions and shows capacity for harboring these conditions prior to death. The combination of proteomics and stable isotope analysis provides a complementary strategy for assessing the demography, diet, lifestyle and possible diseases experienced by ancient populations.
HostingRepository	PRIDE
AnnounceDate	2019-02-28
AnnouncementXML	Submission_2019-02-28_00:24:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valerie Wasinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-06 01:35:03	ID requested
1	2019-02-25 04:13:38	announced
⏵ 2	2019-02-28 00:24:59	announced	Updated project metadata.

Wasinger VC, Curnoe D, Bustamante S, Mendoza R, Shoocongdej R, Adler L, Baker A, Chintakanon K, Boel C, Tacon PSC, Analysis of the Preserved Amino Acid Bias in Peptide Profiles of Iron Age Teeth from a Tropical Environment Enable Sexing of Individuals Using Amelogenin MRM. Proteomics, 19(5):e1800341(2019) [pubmed]

curator keyword: Biological

submitter keyword: tooth, proteome, iron-age

Valerie Wasinger
contact affiliation	Bioanalytical Mass Spectrometry Facility Mark Wainwright Analytical Centre University of NSW Australia
contact email	v.wasinger@unsw.edu.au
lab head
Valerie Wasinger
contact affiliation	UNSW
contact email	v.wasinger@unsw.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009418
     1. Label: PRIDE project
     2. Name: Analysis of the preserved amino acid bias in peptide profiles of Iron Age teeth from a tropical environment enabling sexing of individuals using amelogen in MRM