PXD009417

PXD009417 is an original dataset announced via ProteomeXchange.

Title	Proteomics of MCF7 cells (LC-MS/MS Orbitrap Fusion Tribrid)
Description	We developed RiboLace, a novel method based on a new puromycin-containing molecule, for the isolation of active ribosomes and associate RNAs by means of an antibody-free and tag-free pull-down approach. We compared riboLace translation estimates with protein levels determined by proteomics in MCF7 cells. RiboLace can be easily and rapidly used to obtain a transcriptome-wide snapshot of active translation from eukaryotic in vitro and in vivo systems.
HostingRepository	PRIDE
AnnounceDate	2018-11-21
AnnouncementXML	Submission_2018-11-21_05:14:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Toma Tebaldi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-06 01:34:15	ID requested
⏵ 1	2018-11-21 05:14:41	announced

Clamer M, Tebaldi T, Lauria F, Bernab, ò P, G, ó, mez-Biagi RF, Marchioretto M, Kandala DT, Minati L, Perenthaler E, Gubert D, Pasquardini L, Guella G, Groen EJN, Gillingwater TH, Quattrone A, Viero G, Active Ribosome Profiling with RiboLace. Cell Rep, 25(4):1097-1108.e5(2018) [pubmed]

curator keyword: Technical

submitter keyword: translation, RiboLace, polysome profiling, RNA-seq, POL-seq, MCF7

Gabriella Viero
contact affiliation	Institute of Biophysics, CNR Unit at Trento, Via Sommarive 18, 38123 Povo (Trento), Italy
contact email	gabriella.viero@cnr.it
lab head
Toma Tebaldi
contact affiliation	Yale University
contact email	toma.tebaldi@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009417

PRIDE project URI

• PRIDE
  1. PXD009417
     1. Label: PRIDE project
     2. Name: Proteomics of MCF7 cells (LC-MS/MS Orbitrap Fusion Tribrid)