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PXD009408

PXD009408 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFission yeast NDR/LATS kinase Orb6 regulates exocytosis via phosphorylation of exocyst complex
DescriptionNDR/LATS kinases regulate multiple aspects of cell polarity and morphogenesis from yeast to mammals, but few of their substrates are known. Fission yeast NDR/LATS kinase Orb6 has been proposed to control cell polarity via spatial regulation of Gef1, a guanine nucleotide exchange factor for the small GTPase Cdc42. Here we show that Orb6 plays a critical role as a positive regulator of exocytosis, independent of Gef1. Through Orb6 inhibition in vivo and quantitative global phosphoproteomics, we identify several proteins involved in membrane trafficking as Orb6 targets, and we confirm Sec3 and Sec5, conserved components of the exocyst complex, as substrates of Orb6 both in vivo and in vitro. Our results suggest that Orb6 kinase activity is crucial for exocyst localization to actively-growing cell tips and for exocyst activity during septum dissolution after cytokinesis. We further show that Orb6 phosphorylation of Sec3 serine-201 contributes to exocyst function in parallel with exocyst protein Exo70. We propose that Orb6 contributes to polarized growth by regulating membrane trafficking at multiple levels.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:44:11.482.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKenneth Sawin
SpeciesList scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-05 01:20:02ID requested
12019-01-31 02:33:43announced
22019-06-17 03:29:42announcedUpdated publication reference for PubMed record(s): 30726745.
32024-10-22 04:44:16announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.celrep.2019.01.027;
Tay YD, Leda M, Spanos C, Rappsilber J, Goryachev AB, Sawin KE, Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex. Cell Rep, 26(6):1654-1667.e7(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: cell polarity,Fission yeast, SILAC, phosphoproteomics, Orb6
Contact List
Ken Sawin
contact affiliationUniversity of Edinburgh
contact emailken.sawin@ed.ac.uk
lab head
Kenneth Sawin
contact affiliationUniversity of Edinburgh
contact emailken.sawin@ed.ac.uk
dataset submitter
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