PXD009408

PXD009408 is an original dataset announced via ProteomeXchange.

Title	Fission yeast NDR/LATS kinase Orb6 regulates exocytosis via phosphorylation of exocyst complex
Description	NDR/LATS kinases regulate multiple aspects of cell polarity and morphogenesis from yeast to mammals, but few of their substrates are known. Fission yeast NDR/LATS kinase Orb6 has been proposed to control cell polarity via spatial regulation of Gef1, a guanine nucleotide exchange factor for the small GTPase Cdc42. Here we show that Orb6 plays a critical role as a positive regulator of exocytosis, independent of Gef1. Through Orb6 inhibition in vivo and quantitative global phosphoproteomics, we identify several proteins involved in membrane trafficking as Orb6 targets, and we confirm Sec3 and Sec5, conserved components of the exocyst complex, as substrates of Orb6 both in vivo and in vitro. Our results suggest that Orb6 kinase activity is crucial for exocyst localization to actively-growing cell tips and for exocyst activity during septum dissolution after cytokinesis. We further show that Orb6 phosphorylation of Sec3 serine-201 contributes to exocyst function in parallel with exocyst protein Exo70. We propose that Orb6 contributes to polarized growth by regulating membrane trafficking at multiple levels.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:11.482.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kenneth Sawin
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-05 01:20:02	ID requested
1	2019-01-31 02:33:43	announced
2	2019-06-17 03:29:42	announced	Updated publication reference for PubMed record(s): 30726745.
⏵ 3	2024-10-22 04:44:16	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2019.01.027;

Tay YD, Leda M, Spanos C, Rappsilber J, Goryachev AB, Sawin KE, Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex. Cell Rep, 26(6):1654-1667.e7(2019) [pubmed]

curator keyword: Biological

submitter keyword: cell polarity,Fission yeast, SILAC, phosphoproteomics, Orb6

Ken Sawin
contact affiliation	University of Edinburgh
contact email	ken.sawin@ed.ac.uk
lab head
Kenneth Sawin
contact affiliation	University of Edinburgh
contact email	ken.sawin@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD009408

PRIDE project URI

• PRIDE
  1. PXD009408
     1. Label: PRIDE project
     2. Name: Fission yeast NDR/LATS kinase Orb6 regulates exocytosis via phosphorylation of exocyst complex