PXD009401

PXD009401 is an original dataset announced via ProteomeXchange.

Title	AGO1x stop codon readthrough isoform
Description	Translational readthrough (TR), the elongation of the polypeptide chain by the ribosome beyond the stop codon, was initially observed for viral RNA and more recently reported for yeast and animal transcripts. TR modulates the protein output and diversifies the proteome 1–5. Here, we report that the expression of a TR isoform of Argonaute 1 (AGO1x) is strongly correlated with the proliferative potential of human breast tumors. In contrast to the canonical AGO1 isoform, AGO1x localizes to the nucleus of human cells. Loss of AGO1x impairs the growth of rapidly dividing cells and leads to accumulation of double stranded RNAs with consequent induction of the interferon response and apoptosis. Our data thus uncover a novel function for a mammalian member of the Argonaute protein family, beyond the miRNA effector pathway. As the specific targeting of the AGO1x strongly impacts the proliferation of cancer cells, our study provides a new approach to interfering with tumor growth.
HostingRepository	PRIDE
AnnounceDate	2020-03-19
AnnouncementXML	Submission_2020-03-19_02:56:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009401
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; TMT6plex; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-04 09:19:05	ID requested
⏵ 1	2020-03-19 02:56:27	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: PRM, AGO1, LC-MS

Alexander Schmidt
contact affiliation	Proteomics Core Facility, Biozentrum, University of Basel, Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009401
     1. Label: PRIDE project
     2. Name: AGO1x stop codon readthrough isoform