PXD009397

PXD009397 is an original dataset announced via ProteomeXchange.

Title	Seedling proteome of Arabidopsis thaliana pux10-3 mutant
Description	description: Lipid droplets (LD) of seedlings lacking PUX10 stay significantly smaller during germination than the LDs of wild type seedlings. The observation that the removal of a LD-associated protein from a plant affects LD morphology has been observed previously {Siloto et al., 2006}{Gidda et al., 2016}. Especially the effect of the knock-out of the major structural LD protein Oleosin1 leading to a strongly increased size of LDs described by Siloto et al. might indicate that the abundance of structural LD proteins influences the LD size. Thus, the influence of the absence of PUX10 on the abundance of these proteins was investigated via a proteomic approach. Here, proteins isolated from homogenized seedlings of different lines (Ws-4 and pux10-3) and different developmental stages (wet seeds, and 1 to 3 days after imbibition (dai)) were subjected to tryptic digest and LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2018-08-13
AnnouncementXML	Submission_2018-08-13_10:49:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Till Ischebeck
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-04 08:25:39	ID requested
⏵ 1	2018-08-13 10:49:30	announced

Kretzschmar FK, Mengel LA, M, ü, ller AO, Schmitt K, Blersch KF, Valerius O, Braus GH, Ischebeck T, PUX10 Is a Lipid Droplet-Localized Scaffold Protein That Interacts with CELL DIVISION CYCLE48 and Is Involved in the Degradation of Lipid Droplet Proteins. Plant Cell, 30(9):2137-2160(2018) [pubmed]

curator keyword: Biological

submitter keyword: seedling, Arabidopsis thaliana, lipid droplet proteins, PUX10

Dr. Till Ischebeck
contact affiliation	Department of Plant Biochemistry Georg-August-Universität Göttingen Justus-von-Liebig Weg 11 37077 Göttingen Germany
contact email	tischeb@gwdg.de
lab head
Till Ischebeck
contact affiliation	University of Goettingen
contact email	tischeb@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009397

PRIDE project URI

• PRIDE
  1. PXD009397
     1. Label: PRIDE project
     2. Name: Seedling proteome of Arabidopsis thaliana pux10-3 mutant