PXD009395

PXD009395 is an original dataset announced via ProteomeXchange.

Title	Adaptive response of the sea urchin Paracentrotus lividus to ocean acidification at a volcanic CO2 vent’s system (Mediterranean Sea, Italy)
Description	Ocean acidification is recognized as one of the most pervasive anthropogenic impact on marine life. A variety of responses have been highlighted in different marine organisms ranging from physiology to gene and protein expression. However, most of these studies have been performed in laboratory exposing adults or developmental stages to CO2 enriched seawater. To what extend the information obtained from these in vitro experiments may be extrapolated to a natural environment is questionable. Here, we utilized the Castello volcanic CO2 vents at Ischia as natural laboratory to study the P. lividus population living at the low pH zone (pH~7.8) compared to those of sea urchins living at control sites. Wide-ranging analyses were performed in animals collected at the acidified site, including the monitoring of their position, the determination of the physico-chemical parameters of the coelomic fluid and an in depth characterization of coelomocytes regarding the number and type of cells, Hsp70 expression, redox status and protein expression through de-novo sequencing analyses. In addition, the respiration, nitrogen metabolism, and skeletal mineralogy of urchins from the vent were examined in comparison with those from control animals. Overall these analyses allowed to understand how the sea urchins can thrive in low pH/high CO2.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:37.926.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simona Nonnis
SpeciesList	scientific name: Paracentrotus lividus (Common sea urchin); NCBI TaxID: 7656;
ModificationList	3'-nitro-L-tyrosine; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-04 04:13:17	ID requested
1	2022-02-28 02:59:23	announced
⏵ 2	2023-11-14 08:49:38	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: proteomics, adaptation, Eco-physiology, sea urchins,Ocean acidification, nanoLC-MS/MS

Gabriella Tedeschi
contact affiliation	Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milano, Italy
contact email	gabriella.tedeschi@unimi.it
lab head
Simona Nonnis
contact affiliation	University of Milan
contact email	simona.nonnis@unimi.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009395
     1. Label: PRIDE project
     2. Name: Adaptive response of the sea urchin Paracentrotus lividus to ocean acidification at a volcanic CO2 vent’s system (Mediterranean Sea, Italy)