PXD009392

PXD009392 is an original dataset announced via ProteomeXchange.

Title	Control of seed dormancy and germination by DOG1-AHG1 PP2C Phosphatase complex via binding to heme
Description	Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of the ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.
HostingRepository	PRIDE
AnnounceDate	2018-06-10
AnnouncementXML	Submission_2018-06-10_11:03:10.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009392
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Moresco
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-04 04:10:10	ID requested
⏵ 1	2018-06-10 11:03:11	announced

Nishimura N, Tsuchiya W, Moresco JJ, Hayashi Y, Satoh K, Kaiwa N, Irisa T, Kinoshita T, Schroeder JI, Yates JR, Hirayama T, Yamazaki T, Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme. Nat Commun, 9(1):2132(2018) [pubmed]

curator keyword: Biological

submitter keyword: Abscisic acid, Arabidopsis , seed

Noriyuki Nishimura
contact affiliation	Radiation Breeding Division, Institute of Crop Science,National Agriculture and Food Research Organization, 2425 Kamimurata, Hitachiohmiya, Ibaraki 319-2293, Japan
contact email	nonishi@affrc.go.jp
lab head
James Moresco
contact affiliation	The Salk Institute for Biological Studies
contact email	jmoresco@salk.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009392
     1. Label: PRIDE project
     2. Name: Control of seed dormancy and germination by DOG1-AHG1 PP2C Phosphatase complex via binding to heme