PXD009381

PXD009381 is an original dataset announced via ProteomeXchange.

Title	Chikungunya virus phosphoproteomics
Description	SILAC-labeled MRC-5 cells were seeded in 75-cm2 flasks 1 day before infection with CHIKV-LS3 [1] at MOI 5. One hour post infection (h p.i.), the inoculum was removed and replaced with SILAC DMEM containing 2% dialyzed FBS, 0.280 mM arginine, 0.384 mM lysine, 0.5 mM proline, 25 mM HEPES, 2 mM L-Glutamine and 1% NEAA. At 2, 8, and 12 h p.i., infected and mock-infected cells were harvested for phosphoproteomics analysis by lysis in 4% SDS, 0.1M Tris pH 7.6, followed by heating to 96°C for 10 min. At 12 h p.i,. protein lysates for western blot (WB) analysis were harvested in 4× Laemmli sample buffer (LSB) (100 mM Tris-HCl, pH 6.8, 40% glycerol, 8% SDS, 40 mM DTT, 0,04 mg/ml bromophenol blue) and cells grown on coverslips were fixed in 3% PFA in PBS . The experiment was performed in duplicate with a label swap.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_03:56:10.851.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PA van Veelen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-03 08:16:03	ID requested
⏵ 1	2022-02-28 03:56:11	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Chikungunya virus CHIKV phosphoproteomics

Peter van Veelen
contact affiliation	Center for Proteomics and Metabolomics, Leiden University Medical Center
contact email	p.a.van_veelen@lumc.nl
lab head
PA van Veelen
contact affiliation	LUMC
contact email	p.a.van_veelen@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD009381

PRIDE project URI

• PRIDE
  1. PXD009381
     1. Label: PRIDE project
     2. Name: Chikungunya virus phosphoproteomics