PXD009377 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dataset in support of Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite-host cross talk |
Description | The highly prevalent gut helminth, Ascaris suum, compromise pigs health and reduce farm productivity worldwide. The closely related human parasite, A. lumbricoides, infects more than 800 million people and causes approximately 1.31 million disability-adjusted life years. The humaninfections are often chronic by nature and the parasites have a profound ability to modulate their hosts immune responses. This study provides the first in-depth characterization of extracellular vesicles (EVs) from different developmental stages and body parts of A. suum and their potential role in the host-parasite interplay. The release of EVs during the third larval stage (L3), L4 and adults was demonstrated by Transmission Electron Microscopy, and the uptake of EVs from adult A. suum in intestinal epithelial cells followed by accumulation of RNA in the nucleus by confocal microscopy. Next Generation Sequencing of EV-derived mi/m?RNA identified a number of micro(mi)RNAs from the different A. suum life stages and body parts and potential transcripts of potential host immune targets, such as IL-13, IL-25 and IL-33, were identified. Proteomics of EVs identified several proteins with immunomodulatory properties and other proteins previously shown to be associated with parasite EVs. Furthermore, EVs from A. suum body fluid stimulated the production of the pro-inflammatory cytokines IL-6 and TNF-α in dendritic cells in vitro. Taken together, these results suggest that A. suum EVs and their cargo may play a role in host-parasite interactions. This knowledge may pave the way for novel strategies for helminth infection control and knowledge of their immune modulatory role. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:44:15.907.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Allan Stensballe |
SpeciesList | scientific name: Ascaris suum; NCBI TaxID: 6253; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-04-03 08:13:49 | ID requested | |
1 | 2019-11-12 10:45:38 | announced | |
⏵ 2 | 2024-10-22 04:44:24 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1080/20013078.2019.1578116; |
Hansen EP, Fromm B, Andersen SD, Marcilla A, Andersen KL, Borup A, Williams AR, Jex AR, Gasser RB, Young ND, Hall RS, Stensballe A, Ovchinnikov V, Yan Y, Fredholm M, Thamsborg SM, Nejsum P, provides evidence of parasite-host cross talk. J Extracell Vesicles, 8(1):1578116(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: proteomics, miRNA, extracellular vesicles,Ascaris suum, immunity, host-parasite interactions |
Contact List
Allan Stensballe |
contact affiliation | Laboratory for Medical Massspectroemtry Aalborg University |
contact email | as@hst.aau.dk |
lab head | |
Allan Stensballe |
contact affiliation | Aalborg University |
contact email | as@hst.aau.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009377
- Label: PRIDE project
- Name: Dataset in support of Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite-host cross talk