PXD009375

PXD009375 is an original dataset announced via ProteomeXchange.

Title	Direct visualization of ERAD nanocompartments within the cell
Description	ER-associated degradation (ERAD) is an evolutionarily-conserved quality control mechanism where misfolded proteins are removed from the endoplasmic reticulum (ER) and degraded by the ubiquitin-proteasome system. While many ERAD-mediating proteins have been identified, it is unknown how they are spatially organized within the cell. Using in situ cryo-electron tomography to image the native molecular landscape of Chlamydomonas, we discovered that ERAD proteins are concentrated within ~200 nm cytosolic foci that contact the ER membrane away from the ER-Golgi interface. These ribosome-excluding ERAD nanocompartments consist of a core of clustered proteasomes surrounded by Cdc48. Active proteasomes directly engage with the ER membrane, indicating an additional Cdc48-independent mechanism for extraction of misfolded proteins. Our study reveals that ERAD has precise cellular architecture, which likely enables efficient protein quality control.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:04:48.723.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nagarjuna Nagaraj
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-03 08:13:14	ID requested
1	2020-05-26 11:25:24	announced
⏵ 2	2024-10-22 05:04:49	announced	2024-10-22: Updated project metadata.

Albert S, Wietrzynski W, Lee CW, Schaffer M, Beck F, Schuller JM, Salom, é PA, Plitzko JM, Baumeister W, Engel BD, Direct visualization of degradation microcompartments at the ER membrane. Proc Natl Acad Sci U S A, 117(2):1069-1080(2020) [pubmed]

10.1073/pnas.1905641117;

curator keyword: Biological

submitter keyword: ERAD electron microscopy

Wolfgang Baumeister
contact affiliation	Department of Molecular Structural Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
contact email	baumeist@biochem.mpg.de
lab head
Nagarjuna Nagaraj
contact affiliation	MaxPlanck Institute for Biochemistry, Core facility
contact email	nagaraj@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD009375

PRIDE project URI

• PRIDE
  1. PXD009375
     1. Label: PRIDE project
     2. Name: Direct visualization of ERAD nanocompartments within the cell