PXD009374

PXD009374 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteomic for CDKL5 substrates
Description	Global phosphoproteomic screen to identify the first cellular substrates of CDKL5. CDKL5 knock-out U2OS cells and CDKL5 wt U2OS cells were generated for the TMT-based phosphoproteomic. Thi leads to the identification and further validation of several phosphopetides of MAP1S, CEP131 and CDKL5 itself. The phosphoproteomic analysis allowed the identification of the first cellular substrates for CDKL5 kinase.
HostingRepository	PRIDE
AnnounceDate	2018-09-24
AnnouncementXML	Submission_2019-06-17_03:28:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-03 08:12:55	ID requested
1	2018-09-24 04:05:16	announced
⏵ 2	2019-06-17 03:28:39	announced	Updated publication reference for PubMed record(s): 30266825.

Mu, ñ, oz IM, Morgan ME, Peltier J, Weiland F, Gregorczyk M, Brown FC, Macartney T, Toth R, Trost M, Rouse J, Phosphoproteomic screening identifies physiological substrates of the CDKL5 kinase. EMBO J, 37(24):(2018) [pubmed]

curator keyword: Biological

submitter keyword: phosphoproteomic,TMT, MS2, CDKL5 substrates

Matthias Trost
contact affiliation	Newcastle university, ICAMB Newcastle Upon tyne, United Kingdom
contact email	matthias.trost@ncl.ac.uk
lab head
Matthias Trost
contact affiliation	Newcastle University
contact email	matthias.trost@ncl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009374

PRIDE project URI

• PRIDE
  1. PXD009374
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteomic for CDKL5 substrates