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PXD009356

PXD009356 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative platelet releasate proteome profiling of acute coronary syndrome versus stable coronary artery disease
DescriptionUpon activation, platelets release a host of soluble and vesicular signals, collectively termed the ‘platelet releasate’ (PR). The contents of this PR play a significant role in haemostasis, inflammation, and pathologic sequelae. Despite this, proteomic studies investigating the PR in coronary artery disease have not been performed. We undertook a comparative label-free quantitative (LFQ) proteomic profiling of the 1U/ml thrombin-induced PR from 13 acute coronary syndrome (ACS-STEMI) versus 14 stable angina pectoris patients using a tandem mass spectrometry approach. We identified differentially released platet proteins including tetranectin (CLEC3B), protein disulfide-isomerase-A3 (PDIA3), coagulation factor V (F5) and fibronectin (FN1). Strikingly, all 9 differential proteins were associated with the GO cellular component term ‘extracellular vesicle’ and reduced levels of EVs were detected in plasma of ACS-STEMI patients. Network analysis revealed 3 PR proteins either reduced (F5; FN1) or absent (CLEC3B) in ACS-STEMI patients, which are strongly connected to both the clotting cascade and major druggable targets on platelets. This moderated signature highlights the possible basis of platelet dysfunction in ACS-STEMI and may prove useful for non-invasive risk assessment of the progression of coronary artery disease.
HostingRepositoryPRIDE
AnnounceDate2020-06-05
AnnouncementXMLSubmission_2020-06-05_03:42:53.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaulina Szklanna
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-28 05:22:52ID requested
12020-06-05 03:42:53announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Acute Coronary Syndrome, Platelets, Platelet Releasate, Proteomics, Stable Coronary Artery Disease, LC-MS/MS
Contact List
Patricia Maguire
contact affiliationSBBS, Conway Institute, UCD, Belfield, Dublin
contact emailpatricia.maguire@ucd.ie
lab head
Paulina Szklanna
contact affiliationUniversity College Dublin
contact emailpaulina.szklanna@ucdconnect.ie
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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