PXD009353

PXD009353 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Data in support of Enhanced amyloid beta proteoform detection in an Alzheimer’s disease model by integrated matrix composition enhanced MALDI Imaging and laser capture microdissection
Description	This study has investigated several parameters to obtain increased detection of the amyloid plaque intact protein profile, including a comparison of commonly used MALDI matrices, acid based reduction of signal suppression as well as the combination of strong acid based protein aggregate extraction from laser microdissection plaques to increase detection of proteoforms. Female APPPS1-21 transgenic mice were analyzed by MALDI Imaging with different matrices on a Ultraflextreme MALDI TOF/TOF instrument followed by data analysis in SCiLS Lab software. A combination of formic acid treatment of laser capture microdissected plaques was further utilized to expand the analysis and validation of amyloid plaques.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:21.557.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Allan Stensballe
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	ultraflex

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-28 02:09:41	ID requested
1	2021-03-25 02:28:42	announced
2	2021-03-25 05:19:53	announced	2021-03-25: Updated project metadata.
⏵ 3	2024-10-22 05:20:22	announced	2024-10-22: Updated project metadata.

Publication List

Bastrup J, Birkelund S, Asuni AA, Volbracht C, Stensballe A, Dual strategy for reduced signal-suppression effects in matrix-assisted laser desorption/ionization mass spectrometry imaging. Rapid Commun Mass Spectrom, 33(22):1711-1721(2019) [pubmed]
10.1002/rcm.8521;

Bastrup J, Birkelund S, Asuni AA, Volbracht C, Stensballe A, Dual strategy for reduced signal-suppression effects in matrix-assisted laser desorption/ionization mass spectrometry imaging. Rapid Commun Mass Spectrom, 33(22):1711-1721(2019) [pubmed]

10.1002/rcm.8521;

Keyword List

curator keyword: Biomedical
submitter keyword: Imaging MS, MALDI,Alzheimers disease, biomarkers

curator keyword: Biomedical

submitter keyword: Imaging MS, MALDI,Alzheimers disease, biomarkers

Contact List

Allan Stensballe
contact affiliation	Aalborg University Laboratory for Medical Massspectrometry
contact email	as@hst.aau.dk
lab head
Allan Stensballe
contact affiliation	Aalborg University
contact email	as@hst.aau.dk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD009353
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD009353

PRIDE project URI

Repository Record List

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