PXD009352

PXD009352 is an original dataset announced via ProteomeXchange.

Title	Heat stress-responsive proteomics and phosphoproteomics in spinach
Description	Elevated temperature limits plant growth and reproduction and poses a growing threat to agriculture. Plant heat stress response is highly conserved and fine-tuned in multiple pathways. Spinach (Spinacia oleracea L.) is a cold tolerant but heat sensitive green leafy vegetable. In this study, heat adaptation mechanisms in spinach sibling inbred heat-tolerant line Sp75 were investigated using physiology, proteomics, and phosphoproteomics approaches. The abundance patterns of 911 heat stress-responsive proteins, and phosphorylation level changes of 48 phosphopeptides representing 45 phosphoproteins indicated that heat induced calcium-mediated signaling, ROS homeostasis, endomembrane trafficking, and cross-membrane transport pathways, as well as > 15 transcription regulation factors. Although photosynthesis was inhibited, diverse primary and secondary metabolisms were employed for enhancing thermotolerance, such as glycolysis, pentose phosphate pathway, amino acid metabolism, fatty acid metabolism, nucleotide metabolism, vitamin metabolism, and isoprenoid biosynthesis. These data constitutes a heat stress-responsive metabolic atlas in spinach, which will springboard further investigation into the sophisticated molecular mechanism of plant heat adaptation and inform spinach molecular breeding initiatives.
HostingRepository	PRIDE
AnnounceDate	2018-07-16
AnnouncementXML	Submission_2018-07-16_05:25:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaojun Dai
SpeciesList	scientific name: Spinacia oleracea; NCBI TaxID: 3562;
ModificationList	phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-28 01:48:15	ID requested
⏵ 1	2018-07-16 05:25:13	announced

Zhao Q, Chen W, Bian J, Xie H, Li Y, Xu C, Ma J, Guo S, Chen J, Cai X, Wang X, Wang Q, She Y, Chen S, Zhou Z, Dai S, Proteomics and Phosphoproteomics of Heat Stress-Responsive Mechanisms in Spinach. Front Plant Sci, 9():800(2018) [pubmed]

curator keyword: Biological

submitter keyword: Spinach, leaf, heat stress, proteomics, phosphoproteomics

Shaojun Dai
contact affiliation	Northeast Forestry University
contact email	735425617@qq.com
lab head
Shaojun Dai
contact affiliation	NEFU
contact email	735425617@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009352

PRIDE project URI

• PRIDE
  1. PXD009352
     1. Label: PRIDE project
     2. Name: Heat stress-responsive proteomics and phosphoproteomics in spinach