PXD009351

PXD009351 is an original dataset announced via ProteomeXchange.

Title	A targeted proteomics strategy to identify markers of resistance in pea
Description	Ascochyta blight, caused by Peyronellaea pinodes, is one of major diseases of pea worldwide. Only low levels of resistance are available in pea cultivars. Quantitative Trait Loci associated with resistance to this disease have been identified, but the resistant genes underlying these QTLs are unknown making difficult marker assisted selection. In complex traits involving protein modifications and changes in protein abundance, quantitative protein estimation can be useful as alternative markers for breeding. In this study, we have developed a targeted proteomic strategy to identify protein markers associated with resistance and explore the feasibility of using them in breeding selection. For such purpose, in a first step a list of target peptides was generated based on proteins highly related to P. pinodes resistance by using two genotypes showing extreme behavior. Subsequently, a second experiment was carried out in a population segregating for resistance. Those peptides constitutively more abundant in the resistant lines were selected as potential markers of resistance to P. pinodes. Based on the results obtained, attending to the functions and correlation among the significantly increased proteins in response to Ascochyta blight, we propose a mechanism of defense in pea. As far as we know, this is the first time that such targeted proteomic strategy (shotgun combined data-independent analysis) is used to identify markers of resistance to this disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:20.220.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	M.ANGELES CASTILLEJO
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-28 01:25:48	ID requested
1	2022-02-22 04:06:08	announced
⏵ 2	2024-10-22 05:33:21	announced	2024-10-22: Updated project metadata.

Castillejo M Á, Fondevilla-Aparicio S, Fuentes-Almagro C, Rubiales D, ) in Pea. J Proteome Res, 19(3):1000-1012(2020) [pubmed]

10.1021/acs.jproteome.9b00365;

curator keyword: Biological

submitter keyword: markers, resistance, Ascochyta blight,targeted proteomics, pea

Maria Angeles Castillejo Sanchez
contact affiliation	Institute for Sustainable Agriculture (CSIC) Spain Current address: Dpt. Biochemistry and Molecular Biology, University of Cordoba (Spain)
contact email	bb2casam@uco.es
lab head
M.ANGELES CASTILLEJO
contact affiliation	CSIC
contact email	macastillejo@ias.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009351
     1. Label: PRIDE project
     2. Name: A targeted proteomics strategy to identify markers of resistance in pea