PXD009345

PXD009345 is an original dataset announced via ProteomeXchange.

Title	Human holo-transferrin glycation
Description	Understanding the effect of glycation on the function of transferrin, the systemic iron transporter, is fundamental to fully grasp the mechanisms leading to the loss of iron homeostasis observed in diabetes mellitus (DM). The spontaneous reaction with protein amino groups is one of the main causes of glucose toxicity, but the site specificity of this reaction is still poorly understood. Unbalance of iron metabolism has long been described in DM patients, and one of the main features is the occurrence of non-transferrin-bound iron in the blood serum. The presence of these toxic iron species is observed, despite low transferrin saturation levels. Here in, an in vitro approach was used to study human holo-transferrin glycation in detail. Holo-transferrin was incubated with increasing concentration of glucose (10; 20; 100 and 500 mM) and glycation sites were identified using nano-reverse phase – liquid chromatography coupled to high resolution mass spectrometry (nLC-MS). Overall 21 glycation hotspots were identified with lysine residues 103, 312 and 380 proving to be the most reactive sites. Glycation specificity was found to be remarkably different from that described for apo-transferrin.
HostingRepository	PRIDE
AnnounceDate	2018-05-30
AnnouncementXML	Submission_2018-06-05_04:17:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	André Silva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N6-carboxymethyl-L-lysine; N6-1-carboxyethyl-L-lysine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-27 08:51:39	ID requested
1	2018-05-30 09:07:09	announced
⏵ 2	2018-06-05 04:17:11	announced	Updated publication reference for PubMed record(s): 29860209.

Silva AMN, Coimbra JTS, Castro MM, Oliveira Â, Br, á, s NF, Fernandes PA, Ramos MJ, Rangel M, Determining the glycation site specificity of human holo-transferrin. J Inorg Biochem, 186():95-102(2018) [pubmed]

ProteomeXchange project tag: Diabetes (B/D-HPP)

curator keyword: Biological, Biomedical

submitter keyword: Glycation, blood serum, holo-transferrin, post-translational modification, LC-MS/MS

André Moreira Neto da Silva
contact affiliation	REQUIMTE-LAQV, DEP. DE QUÍMICA E BIOQUÍMICA, FACULDADE DE CIÊNCIAS DA UNIVERSIDADE DO PORTO, RUA DO CAMPO ALEGRE, S/N, 4169-007 PORTO (PORTUGAL)
contact email	andre.silva@fc.up.pt
lab head
André Silva
contact affiliation	REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Portugal
contact email	andre.silva@fc.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD009345

PRIDE project URI

• PRIDE
  1. PXD009345
     1. Label: PRIDE project
     2. Name: Human holo-transferrin glycation