PXD009335

PXD009335 is an original dataset announced via ProteomeXchange.

Title	The FAM83 family of proteins are anchors for Casein Kinase 1 isoforms through the DUF1669 domain
Description	The Casein Kinase 1 (CK1) family of Ser/Thr protein kinases are implicated in the regulation of many cellular processes, including cell cycle, circadian rhythm, Wnt and Sonic Hedgehog signalling. Regarded as constitutively active kinases, their regulation in cells is critically important but poorly understood. We report here that members of the FAM83 family of uncharacterized proteins are central regulators of CK1 isoforms in cells. The eight members of the FAM83 family (A-H) interact and co-localize with different CK1 alpha, alpha-like, delta and epsilon isoforms. We demonstrate that the interaction with CK1 isoforms is mediated through a number of residues within a conserved domain of unknown function, termed DUF1669, which characterises the FAM83 family. CK1-binding deficient mutants of FAM83 proteins interfere with the subcellular localization of CK1 isoforms as well as FAM83 proteins themselves and their cellular functions. We propose that DUF1669 be renamed the Polypeptide Anchor of CK1 (PACK1) domain.
HostingRepository	PRIDE
AnnounceDate	2020-04-16
AnnouncementXML	Submission_2020-04-16_01:53:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gopal Sapkota
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-26 07:50:11	ID requested
1	2018-05-24 06:37:09	announced
⏵ 2	2020-04-16 02:01:21	announced	2020-04-16: Updated publication reference for PubMed record(s): 32289446.

Tachie-Menson T, G, á, zquez-Guti, é, rrez A, Fulcher LJ, Macartney TJ, Wood NT, Varghese J, Gourlay R, Soares RF, Sapkota GP, Characterisation of the biochemical and cellular roles of native and pathogenic amelogenesis imperfecta mutants of FAM83H. Cell Signal, 72():109632(2020) [pubmed]

curator keyword: Biological

submitter keyword: FAM83 Proteomics

Gopal P Sapkota
contact affiliation	University of Dundee
contact email	g.sapkota@dundee.ac.uk
lab head
Gopal Sapkota
contact affiliation	University of Dundee
contact email	g.sapkota@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009335
     1. Label: PRIDE project
     2. Name: The FAM83 family of proteins are anchors for Casein Kinase 1 isoforms through the DUF1669 domain