PXD009333

PXD009333 is an original dataset announced via ProteomeXchange.

Title	Fam20C-LCMSMS - Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1α
Description	Family with sequence similarity 20C (Fam20C), the physiological Golgi casein kinase, phosphorylates numerous secreted proteins that are involved in a wide variety of biological processes. However, the role of Fam20C in regulating proteins in the endoplasmic reticulum (ER) lumen is largely unknown. Here we report that Fam20C interacts with various luminal proteins and that its depletion results in a more reduced ER lumen. We further show that ER oxidoreductin 1α (Ero1α), the pivotal sulfhydryl oxidase that catalyzes disulfide formation in the ER, is phosphorylated at Ser145 under reductive stress and under secretion-demanding conditions, such as mammalian lactation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:52.596.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianchao Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-26 07:33:10	ID requested
1	2018-05-30 09:16:59	announced
2	2018-06-05 08:24:56	announced	Updated publication reference for PubMed record(s): 29858230.
⏵ 3	2024-10-22 04:44:00	announced	2024-10-22: Updated project metadata.

Zhang J, Zhu Q, Wang X, Yu J, Chen X, Wang J, Wang X, Xiao J, Wang CC, Wang L, . EMBO J, 37(14):(2018) [pubmed]

10.15252/embj.201798699;

curator keyword: Biological

submitter keyword: Fam20C Ero1 LCMSMS

Lei Wang
contact affiliation	National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
contact email	wanglei@moon.ibp.ac.cn
lab head
Jianchao Zhang
contact affiliation	Institue of Biophysics, Chinese Academy of Sciences
contact email	zhangjianchao@moon.ibp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD009333

PRIDE project URI

• PRIDE
  1. PXD009333
     1. Label: PRIDE project
     2. Name: Fam20C-LCMSMS - Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1α