PXD009331

PXD009331 is an original dataset announced via ProteomeXchange.

Title	Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa
Description	Cryopreservation causes significant lethal and sub-lethal damage to spermatozoa. In order to improve freezing outcomes, a comprehensive understanding of sub-lethal damage is required. Cryopreservation induced changes to sperm proteins have been investigated in several species, but few have employed currently available state of the art, data independent acquisition mass spectrometry (MS) methods. We used the SWATH LC-MS method to quantitatively profile proteomic changes to ram spermatozoa following exposure to egg yolk and cryopreservation. Egg yolk contributed 15 proteins to spermatozoa, including vitellogenins, apolipoproteins and complement component C3. Cryopreservation significantly altered the abundance of 51 proteins. Overall, 27 proteins increased (e.g. SERPINB1, FER) and 24 proteins decreased (e.g. CCT subunits, CSNK1G2, TOM1L1) in frozen thawed ram spermatozoa, compared to fresh spermatozoa. Chaperones constituted 20% of the proteins lost from spermatozoa following cryopreservation. These alterations may interfere with both normal cellular functioning and the ability of frozen thawed spermatozoa to appropriately respond to stress. This is the first study to apply SWATH mass spectrometry techniques to characterise proteins contributed by egg yolk based freezing media and to profile cryopreservation induced proteomic changes to ram spermatozoa.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:54.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taylor Pini
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-26 03:08:26	ID requested
1	2018-04-26 02:36:01	announced
⏵ 2	2024-10-22 04:44:02	announced	2024-10-22: Updated project metadata.

Pini T, Rickard JP, Leahy T, Crossett B, Druart X, de Graaf SP, Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa. J Proteomics, 181():73-82(2018) [pubmed]

10.1016/j.jprot.2018.04.001;

curator keyword: Technical

submitter keyword: SWATH, sperm,Ram

Taylor Pini
contact affiliation	School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Australia
contact email	taylor.pini@sydney.edu.au
lab head
Taylor Pini
contact affiliation	University of Sydney
contact email	tpini@flcolo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD009331

PRIDE project URI

• PRIDE
  1. PXD009331
     1. Label: PRIDE project
     2. Name: Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa