PXD009324

PXD009324 is an original dataset announced via ProteomeXchange.

Title	3D-Printed Device for Consistent and High-Throughput Peptide Sample Clean-up Using StageTips
Description	Mass spectrometry (MS) is a fundamental technology for the study of proteomics. A core technique for MS analysis is solid-phase extraction (SPE) for the removal of contaminants and sample concentration, and StageTips are one of the most commonly used devices used for SPE in modern proteomics workflows. Here, we detail a device that can accommodate up to 96 StageTips enabling high-speed centrifugal-based processing of StageTips, including collection into PCR tubes, or 96-well plates, simplifying the processing of samples for MS analysis. The device can be rapidly and economically manufactured using a 3D printer employing fused deposition modelling. We show that high impact polystyrene (HIPS) is a particularly suitable material for building this device, providing good chemical resistance and is able to withstand high centrifugal speeds to enable repeated use. We provide detailed schematics as well as the corresponding CAD files for the community, enabling reproduction of the device on any common 3D printer model. Using an optimized protocol, we show that our tip spinner device provides similar peptide identifications and comparable inter-sample consistency than previously established centrifugation-based cleanup on individual StageTips. As such, this apparatus facilitates greater throughput in sample preparation for mass spectrometry, without detracting from sample quality.
HostingRepository	PRIDE
AnnounceDate	2019-03-21
AnnouncementXML	Submission_2019-03-21_09:55:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Larance
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-26 02:24:03	ID requested
⏵ 1	2019-03-21 09:55:14	announced

Harney DJ, Hutchison AT, Hatchwell L, Humphrey SJ, James DE, Hocking S, Heilbronn LK, Larance M, Proteomic Analysis of Human Plasma during Intermittent Fasting. J Proteome Res, 18(5):2228-2240(2019) [pubmed]

curator keyword: Technical

submitter keyword: StageTip, 3D-Printed, 96-well

Mark Larance
contact affiliation	The University of Sydney, School of Life and Environmental Sciences, Charles Perkins Centre, Sydney, New South Wales, Australia.
contact email	mark.larance@sydney.edu.au
lab head
Mark Larance
contact affiliation	The University of Sydney
contact email	mark.larance@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD009324

PRIDE project URI

• PRIDE
  1. PXD009324
     1. Label: PRIDE project
     2. Name: 3D-Printed Device for Consistent and High-Throughput Peptide Sample Clean-up Using StageTips