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PXD009320

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-07-11
  • AnnouncementXML: Submission_2018-07-11_09:43:13.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Mirele Daiana Poleti
  • Title: Bovine Longissimus thoracis muscle HDMSE with differents ultimate pH
  • Description: The purpose of this research was to investigate the causes and consequences of pHu variations in beef cattle. For that, were evaluated 176 Nellore beef cattle, and classified into two different pHu groups: High (≥6.0, N=17) and Normal (<5.8, N=159). Plasma concentrations of cortisol and adrenocorticotropic hormone, lactate and glycogen muscular content, meat color, shear force and Longissimus thoracis muscle proteomic profile were evaluated and compared between pHu groups. Muscle glycogen content, meat color and shear force statistically differed between pHu groups. Label-free quantitative proteomic analysis revealed ten differentially abundant proteins between pHu groups, involved in metabolic processes and muscle contraction. Thirty-six and 31 proteins were exclusively present in Normal and High pHu group, respectively, which were related to TCA cycle, cortisol production, calcium regulation, and antioxidant function. The MYH7, UGP2, and VDAC3 were identified as potential indicators of pHu variations. CALM and NNT appeared to be interesting proteins to better understand the metabolic pathways behind pHu.
  • SpeciesList: scientific name: Bos indicus; NCBI TaxID: 9915;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: Synapt MS

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-03-26 01:25:24ID requested
12018-07-11 09:43:14announced

Publication List

  1. Poleti MD, Moncau CT, Silva-Vignato B, Rosa AF, Lobo AR, Cataldi TR, Negrão JA, Silva SL, Eler JP, de Carvalho Balieiro JC, Label-free quantitative proteomic analysis reveals muscle contraction and metabolism proteins linked to ultimate pH in bovine skeletal muscle. Meat Sci, 145():209-219(2018) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: HDMSE, muscle, bovine, ultimate pH

Contact List

    Julio César de Carvalho Balieiro
    • contact affiliation: College of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil.
    • contact email: balieiro@usp.br
    • lab head:
    Mirele Daiana Poleti
    • contact affiliation: University of São Paulo
    • contact email: mdpoleti@gmail.com
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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