PXD009319 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain |
Description | The current work aimed to detect and identify significantly differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which were in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all theoretical spectra (SWATH) MS as tools on the two well-defined strains. In the gel-based experiments, 49 identified protein spots were found to be differentially expressed, of which 37 belonged to the in vitro cultivated virulent strain and 12 to the attenuated one. The most frequently identified proteins in the virulent strain take part in cytoskeleton formation, carbohydrate metabolism and adaptation to stress. However, post-translationally modified or truncated ubiquitous cellular proteins such as actin and GAPDH were identified as upregulated in multiple gel positions. This indicated their contribution to processes not related to cytoskeleton and carbohydrate metabolism, such as fibronectin or plasminogen binding. On the other hand, proteins involved in cytoskeleton, cell division and cytoskeleton organization were frequently observed in the attenuated strain. The findings of the gel-based studies were supplemented by the gel-free SWATH MS analysis, which identified and quantified 42 significantly differentially regulated proteins. In this case proteins with peptidase activity, metabolic proteins and actin-regulating proteins were the most frequent findings in the virulent strain, while proteins involved in hydrogenosomal carbohydrate metabolism dominated the results in the attenuated one. |
HostingRepository | PRIDE |
AnnounceDate | 2018-11-21 |
AnnouncementXML | Submission_2018-12-10_02:23:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ivana BILIC |
SpeciesList | scientific name: Histomonas meleagridis; NCBI TaxID: 135588; |
ModificationList | No PTMs are included in the dataset |
Instrument | liquid chromatography separation; TripleTOF 5600+ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-03-26 00:46:20 | ID requested | |
1 | 2018-11-21 05:29:38 | announced | |
⏵ 2 | 2018-12-10 02:23:32 | announced | Updated publication reference for PubMed record(s): 30505807. |
Publication List
Monoyios A, Hummel K, N, รถ, bauer K, Patzl M, Schlosser S, Hess M, Bilic I, Strain. Front Cell Infect Microbiol, 8():407(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Histomonas meleagridis, protozoa, comparative proteomic analysis, 2D-DIGE, MALDI-TOF/TOF, LC-MS/MS, SWATH MS, virulence factors, attenuation. |
Contact List
Dr. Ivana Bilic |
contact affiliation | University Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria |
contact email | ivana.bilic@vetmeduni.ac.at |
lab head | |
Ivana BILIC |
contact affiliation | Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna |
contact email | ivana.bilic@vetmeduni.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009319 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009319
- Label: PRIDE project
- Name: An alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain