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PXD009316

PXD009316 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
DescriptionThe current work aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which were in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all theoretical spectra (SWATH) MS as tools on the two well-defined strains. In the gel-based experiments, 49 identified protein spots were found to be differentially expressed, of which 37 belonged to the in vitro cultivated virulent strain and 12 to the attenuated one. The most frequently identified proteins in the virulent strain take part in cytoskeleton formation, carbohydrate metabolism and adaptation to stress. However, post-translationally modified or truncated ubiquitous cellular proteins such as actin and GAPDH were identified as upregulated in multiple gel positions. This indicated their contribution to processes not related to cytoskeleton and carbohydrate metabolism, such as fibronectin or plasminogen binding. On the other hand, proteins involved in cytoskeleton, cell division and cytoskeleton organization were frequently observed in the attenuated strain. The findings of the gel-based studies were supplemented by the gel-free SWATH MS analysis, which identified and quantified 42 significantly differentially regulated proteins. In this case proteins with peptidase activity, metabolic proteins and actin-regulating proteins were the most frequent findings in the virulent strain, while proteins involved in hydrogenosomal carbohydrate metabolism dominated the results in the attenuated one.
HostingRepositoryPRIDE
AnnounceDate2018-12-10
AnnouncementXMLSubmission_2018-12-10_03:25:17.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIvana BILIC
SpeciesList scientific name: Histomonas meleagridis; NCBI TaxID: 135588;
ModificationList2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrumentliquid chromatography separation; TripleTOF 5600+; Nanoelectrospray
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-26 00:28:58ID requested
12018-12-10 03:25:18announced
Publication List
Monoyios A, Hummel K, N, รถ, bauer K, Patzl M, Schlosser S, Hess M, Bilic I, Strain. Front Cell Infect Microbiol, 8():407(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Histomonas meleagridis, protozoa, comparative proteomic analysis, 2D-DIGE, MALDI-TOF/TOF, LC-MS/MS, SWATH MS, virulence factors, attenuation.
Contact List
Dr. Ivana Bilic
contact affiliationUniversity Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria
contact emailivana.bilic@vetmeduni.ac.at
lab head
Ivana BILIC
contact affiliationClinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna
contact emailivana.bilic@vetmeduni.ac.at
dataset submitter
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