PXD009311

PXD009311 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics analysis of normal and mutated granulocyte colony stimulating factor receptors
Description	Granulocyte colony stimulating factor receptor (G-CSFR) plays important role in the production of neutrophils from hematopoietic stem cells. Mutated form of the receptor has been directly associated with two distinct malignant phenotype in patients, e.g. acute myeloid leukemia (AML) and chronic neutrophilic leukemia (CNL). However, the signaling mechanism of the mutated G-CSFRs is not well understood. Here, we describe a comprehensive SILAC based quantitative phosphoproteomic analysis of the mutated G-CSFRs compared to the normal receptor using BaF3 cell line based in vitro model system. High pH reversed phase concatenation and Titanium Dioxide Spin Tip column were utilized to increase the dynamic range and detection of the phosphoproteome of G-CSFRs. The dataset was further analyzed using several computational and bioinformatics tools. Overall, this dataset is a first of any phosphoproteomics analysis of granulocyte colony stimulating factor receptors in the normal and disease associated mutations. We anticipate that our dataset will have a strong potential to decipher the phospho-signaling differences between the normal and malignant G-CSFR biology with therapeutic implications.
HostingRepository	PRIDE
AnnounceDate	2019-02-14
AnnouncementXML	Submission_2019-06-17_03:28:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pankaj Dwivedi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-23 09:15:56	ID requested
1	2019-02-14 12:11:48	announced
⏵ 2	2019-06-17 03:28:17	announced	Updated publication reference for PubMed record(s): 30967555.

Dwivedi P, Muench DE, Wagner M, Azam M, Grimes HL, Greis KD, Phospho serine and threonine analysis of normal and mutated granulocyte colony stimulating factor receptors. Sci Data, 6(1):21(2019) [pubmed]

curator keyword: Biological

submitter keyword: Phosphoproteomics, G-CSFR signaling, SILAC

Kenneth D. Greis
contact affiliation	Department of Cancer Biology, University of Cincinnati, Cincinnati, Ohio 45267, USA
contact email	greiskd@ucmail.uc.edu
lab head
Pankaj Dwivedi
contact affiliation	University of Cincinnati
contact email	dwivedpj@mail.uc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD009311

PRIDE project URI

• PRIDE
  1. PXD009311
     1. Label: PRIDE project
     2. Name: Phosphoproteomics analysis of normal and mutated granulocyte colony stimulating factor receptors