PXD009310

PXD009310 is an original dataset announced via ProteomeXchange.

Title	The platelet releasate in healthy population and pregancy
Description	Upon activation, platelets release a multitude of soluble and vesicular signals, collectively termed the ‘platelet releasate’ (PR). This PR plays an important role in haemostasis, wound healing and the inflammatory response. We and others have used qualitative/quantitative proteomic approaches to characterise the PR; however, with reports of marked inter-individual variability, confident and reliable insights have been hindered. Here we aimed to provide a reproducible and quantifiable proteomic analysis and establish a 'core' human PR, as well as to assess its variability in healthy human pregnancy where increased platelet activation is observed. 896 proteins from thrombin-induced PRs were quantified across 32healthy donors, with 277 proteins forming a ‘core’ PR with low variability. All 277 core PR proteins were identified in pregnancy. Furthermore 69 proteins were found differentially released from platelets in healthy human pregnancy. In summary, the PR comprises a ‘core’ set of proteins between healthy individuals. This dynamic proteome significantly changes in physiologic and pathologic conditions. Thus, the PR is a barcode for the health status of an individual at a given time.
HostingRepository	PRIDE
AnnounceDate	2018-06-25
AnnouncementXML	Submission_2018-06-25_06:23:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulina Szklanna
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-23 09:08:34	ID requested
⏵ 1	2018-06-25 06:23:01	announced

Parsons MEM, Szklanna PB, Guerrero JA, Wynne K, Dervin F, O'Connell K, Allen S, Egan K, Bennett C, McGuigan C, Gheveart C, N, í, , Á, inle F, Maguire PB, Platelet Releasate Proteome Profiling Reveals a Core Set of Proteins with Low Variance between Healthy Adults. Proteomics, 18(15):e1800219(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, platelets, platelet releasate, pregnancy, LC-MS/MS

Patricia Maguire
contact affiliation	SBBS, Conway Institute, UCD, Belfield, Dublin
contact email	patricia.maguire@ucd.ie
lab head
Paulina Szklanna
contact affiliation	University College Dublin
contact email	paulina.szklanna@ucdconnect.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009310

PRIDE project URI

• PRIDE
  1. PXD009310
     1. Label: PRIDE project
     2. Name: The platelet releasate in healthy population and pregancy