PXD009309

PXD009309 is an original dataset announced via ProteomeXchange.

Title	Human RPE LC-MSMS .
Description	AMD is a complex disorder related to environmental factors and genetic polymorphisms. We explored the interplay between the two factors by comparing the differences between HTRA1/ARMS2 high-risk and low-risk AMD alleles after exposure to smokers’ serum using iTRAQ-based proteomics. Primary human retinal pigment epithelial (hRPE) cells were obtained from donor eyes and screened for ARMS2/HTRA1 AMD risk genotypes, wild type allele as control. A gene ontology annotation analysis and KEGG metabolic pathway analysis were employed to obtain a global functional view of differentially expressed (DE) proteins. Significant DE proteins were screened and validated by real-time PCR, immunofluorescence and western blot. The relationship between HTRA1 and caveolin-1 protein was explored by HTRA1-overexpressing lentivirus-transduced ARPE-19 cells. A total of 400 DE proteins were detected for the high-risk allele after cigarette sera stimulation, which was much higher than the low-risk allele. The caveolin-1 protein was significantly up-regulated when HTRA1-overexpressing cells were exposed to smokers’ serum and the smoking effect was stronger than AMD high-risk allele. There were interplays between AMD high-risk allele and cigarette smoking. Smokers’ sera also accelerated the phagocytosis of hRPE cells. In addition, caveolin-1 may play an important role in the disease process, but its molecular mechanism requires further study.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:12:03.690.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bincui Cai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-23 06:21:00	ID requested
⏵ 1	2024-10-07 10:12:04	announced

10.1167/tvst.11.2.15;

Cai B, Zhang Z, Sun S, Lin T, Ke Y, Li Z, Yang J, Li X, A Pilot Application of an iTRAQ-Based Proteomics Screen Estimates the Effects of Cigarette Smokers' Serum on RPE Cells With AMD High-Risk Alleles. Transl Vis Sci Technol, 11(2):15(2022) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, LC-MSMS,retinal, RPE

Xiaorong Li
contact affiliation	Tianjin Medical University Eye Hospital, Tianjin Medical University Ophthalmology Research Institute，Tianjin,300384
contact email	xiaorli@163.com
lab head
Bincui Cai
contact affiliation	Tianjin medical university eye hosipital
contact email	516117732@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009309
     1. Label: PRIDE project
     2. Name: Human RPE LC-MSMS