PXD009308

PXD009308 is an original dataset announced via ProteomeXchange.

Title	Identification of protein-trichloroethene interactions during organohalide respiration revealed by thermal proteome profiling
Description	The organohalide-respiring Sulfurospirillum multivorans uses chlorinated ethenes as electron acceptors for growth under anoxic conditions. However, little is known about the interaction of these substrates with proteins. Here, we apply thermal proteome profiling (TPP) to analyze enzyme-trichloroethene interactions. TPP is commonly used to investigate protein-ligand binding through protein melting curve shifts. Several modifications in the protocol, e.g. performing the incubation under anaerobic conditions and increasing the temperature range up to 97°C, improved the detection range and allowed the investigation of oxygen-sensitive proteins. Enzymatic reductive dehalogenation was prevented by omitting the electron donor during incubations. This enabled detecting the interaction of the tetrachloroethene reductive dehalogenase PceA with trichloroethene and confirms the enzyme’s specificity for this substrate. Another 19 proteins showed significant melting curve shifts with trichloroethene, pointing to other proteins directly or indirectly interacting with trichloroethene. Interestingly, a putative response regulator reacted similarly towards trichloroethene, which is potentially in line with its proposed role in regulating trichloroethene respiration. The TPP approach is here proven to facilitate the identification of substrate-enzyme interactions of strictly anaerobic reductive dehalogenases and probably their regulators. This strategy can be used to identify yet unknown substrate specificities and potential signal-sensing proteins in other difficult to study bacteria.
HostingRepository	PRIDE
AnnounceDate	2018-06-06
AnnouncementXML	Submission_2018-06-28_00:56:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominique Türkowsky
SpeciesList	scientific name: Sulfurospirillum multivorans DSM 12446; NCBI TaxID: 1150621;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-23 06:12:19	ID requested
1	2018-06-06 02:09:16	announced
⏵ 2	2018-06-28 00:56:52	announced	Updated publication reference for PubMed record(s): 29879467.

T, ü, rkowsky D, Lohmann P, M, ü, hlenbrink M, Schubert T, Adrian L, Goris T, Jehmlich N, von Bergen M, Thermal proteome profiling allows quantitative assessment of interactions between tetrachloroethene reductive dehalogenase and trichloroethene. J Proteomics, 192():10-17(2019) [pubmed]

curator keyword: Biological

submitter keyword: Thermal proteome profiling (TPP)

Interaction proteome

organohalide respiration

reductive dehalogenases

Sulfurospirillum multivorans

Dehalococcoides mccartyi

quantitative proteomics

Martin von Bergen
contact affiliation	Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research – UFZ, Permoserstraße 15, 04318 Leipzig, Germany
contact email	martin.vonbergen@ufz.de
lab head
Dominique Türkowsky
contact affiliation	Helmholtz Centre for Environmental Research - UFZ
contact email	dominique.tuerkowsky@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009308

PRIDE project URI

• PRIDE
  1. PXD009308
     1. Label: PRIDE project
     2. Name: Identification of protein-trichloroethene interactions during organohalide respiration revealed by thermal proteome profiling