PXD009303

PXD009303 is an original dataset announced via ProteomeXchange.

Title	Urinary proteome in diabetic nephropathy
Description	Urinary proteome was analyzed and quantified by Tandem Mass Tag (TMT) labeling followed by bioinformatics analysis to study DN pathophysiology and to identify biomarkers of clinical outcome. We included type 2 diabetic normotensive non-obese males with and without incipient DN (microalbuminuria). Sample collection included blood and urine at baseline (control and DN-basal) and, in DN patients, after 3 months of losartan treatment (DN-treated). Urinary proteome analysis identified 166 differentially abundant proteins between controls and DN patients, 27 comparing DN-treated and DN-basal patients, and 182 between DN-treated patients and controls. Mathematical modeling analysis identified 80 key proteins involved in DN pathophysiology, 15 in losartan effect and 7 of these 95 are essential in both processes. VCAM-1 and neprilysin are the only ones that are differentially expressed in the urinary proteome. We observed an increase of VCAM-1 urine levels in DN-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria, the latter confirmed by ELISA . Our results point to neprilysin and VCAM-1 as potential candidates in DN pathology and treatment.
HostingRepository	PRIDE
AnnounceDate	2018-05-22
AnnouncementXML	Submission_2018-05-22_04:24:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Montserrat Carrascal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-23 04:49:55	ID requested
⏵ 1	2018-05-22 04:24:11	announced

Checa-Rojas A, Delgadillo-Silva LF, Velasco-Herrera MDC, Andrade-Dom, í, nguez A, Gil J, Santill, á, n O, Lozano L, Toledo-Leyva A, Ram, í, rez-Torres A, Talamas-Rohana P, Encarnaci, ó, n-Guevara S, GSTM3 and GSTP1: novel players driving tumor progression in cervical cancer. Oncotarget, 9(31):21696-21714(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, urinary proteome, diabetes, quantitative proteomics

Montserrat Carrascal
contact affiliation	Proteomics Laboratory CSIC/UAB, Institut d’Investigacions Biomèdiques de Barcelona (IIBB-CSIC-IDIBAPS), Barcelona, Spain.
contact email	montserrat.carrascal.csic@uab.cat
lab head
Montserrat Carrascal
contact affiliation	IIBB-CSIC
contact email	montserrat.carrascal.csic@uab.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD009303

PRIDE project URI

• PRIDE
  1. PXD009303
     1. Label: PRIDE project
     2. Name: Urinary proteome in diabetic nephropathy