PXD009295

PXD009295 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	I/L-discrimination by MS3 of dimethylated peptides
Description	A novel mass spectrometric method for discriminating isoleucine and leucine by MS3. The dimethylation of peptide N-termini leads to intense a1 ions in CID and MS3 fragmentation of the isoleucine/leucine a1-ion leads to spectra with fragments that can discriminate between the two isomers. The method was applied to two antibodies in combination with a set of protease treatments using trypsin, thermolysin, chymotrypsin and pepsin to generate peptides exposing N-terminal I/L residues.
HostingRepository	PRIDE
AnnounceDate	2018-07-24
AnnouncementXML	Submission_2018-07-24_09:40:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009295
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jakob Bunkenborg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Glu->pyro-Glu; Deamidated; Dimethyl; Carbamidomethyl; Oxidation; Dimethyl:2H(4)
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-22 02:41:34	ID requested
⏵ 1	2018-07-24 09:40:20	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

curator keyword: Technical
submitter keyword: LC-MS, isoleucine, leucine, I/L-discrimination, Orbitrap Fusion, MS3, Antibody

curator keyword: Technical

submitter keyword: LC-MS, isoleucine, leucine, I/L-discrimination, Orbitrap Fusion, MS3, Antibody

Contact List

Jakob Bunkenborg
contact affiliation	ALPHALYSE A/S Unsbjergvej 4 • 5220 Odense SØ • Denmark Phone: +45 6310 6505
contact email	bunkenborg@alphalyse.com
lab head
Jakob Bunkenborg
contact affiliation	Alphalyse
contact email	jakob.bunkenborg@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009295
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009295

PRIDE project URI

Repository Record List

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