PXD009294

PXD009294 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins that interact with extracellularly applied fibrillar Tau assemblies
Description	The microtubule associated protein Tau (MAPT) expressed in neurons is involved in microtubules stabilization, cell morphogenesis and axonal transport. In pathological conditions, Tau assembles into high molecular weight assemblies leading to neuropathological Tau deposits, the hallmark of several neurodegenerative diseases collectively named “tauopathies”, including Alzheimer’s disease. These pathologic Tau assemblies are released by affected neuronal cells and taken up by naïve neighbor cells, propagate from one cell to another and amplify by seeding the aggregation of endogenous Tau. In order to identify plasma membrane proteins exposed extracellularly that interact with extracellularly applied fibrillar Tau assemblies, we exposed pure-neuronal cultures to fibrillar Tau for 10 min, pulled down the associated proteins, and identified them using a proteomic-based approach. Of the six Tau isoforms produced by alternative splicing of the MAPT gene and differing from each other by the presence or absence of one or two inserts in the N-terminal part (0N, 1N or 2N) of the protein and by the presence of either three or four repeated microtubule binding motifs in the protein C-terminal part (3R or 4R), we have expressed and purified 1N3R and 1N4R Tau isoforms and further assembled them into 1N3R and 1N4R Tau fibrils. Using pull-down of whole cell lysates and mass spectrometry, we have identified proteins interacting with extracellularly applied Tau fibrils. We have performed two different experiments with either 1N3R Tau fibrils or 1N4R Tau fibrils (condition 1 and condition 2 respectively). Each condition consists in six experimental replicates of cells exposed 10 min to Tau fibrils and of the non-treated cells used as controls.
HostingRepository	PRIDE
AnnounceDate	2019-01-09
AnnouncementXML	Submission_2019-01-14_04:40:47.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009294
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Virginie Redeker
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Glu->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	TripleTOF 4600

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-22 02:36:35	ID requested
1	2019-01-09 08:39:31	announced
⏵ 2	2019-01-14 04:40:49	announced	Updated publication reference for PubMed record(s): 30630857.

Shrivastava AN, Redeker V, Pieri L, Bousset L, Renner M, Madiona K, Mailhes-Hamon C, Coens A, Bu, é, e L, Hantraye P, Triller A, Melki R, -ATPase and AMPA receptors. EMBO J, 38(3):(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Tau fibrils, 1N3R isoform, 1N4R isoform, Interacting protein partners, Pull-down, LC-MSMS, Proteomics

Ronald Melki, Virginie Redeker
contact affiliation	Paris-Saclay Institute of Neuroscience, and CEA, Institut François Jacob, Université Paris-Saclay, Team Melki, CNRS, Gif-sur-Yvette, France
contact email	virginie.redeker@cnrs.fr
lab head
Virginie Redeker
contact affiliation	CNRS
contact email	virginie.redeker@cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009294
     1. Label: PRIDE project
     2. Name: Identification of proteins that interact with extracellularly applied fibrillar Tau assemblies