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PXD009291

PXD009291 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing
DescriptionRegular blood transfusion is the cornerstone of care for patients with red blood cell (RBC) disorders such as thalassemia or sickle cell disease. With repeated transfusion, alloimmunisation often occurs due to incompatibility at the level of minor blood group antigens. We use CRISPR-mediated genome editing of an immortalised human erythroblast cell line (BEL-A) to generate multiple enucleation competent cell lines deficient in individual blood groups. Edits are combined to generate a single cell line deficient in multiple antigens responsible for the most common transfusion incompatibilities: ABO (Bombay phenotype), Rh (Rhnull), Kell (K0), Duffy (Duffynull), GPB (S- s- U-). These cells can be differentiated to generate deformable reticulocytes, illustrating the capacity for coexistence of multiple rare blood group antigen null phenotypes. This study provides the first proof-of-principle demonstration of combinatorial CRISPR-mediated blood group gene editing to generate customisable or multi-compatible RBCs for diagnostic reagents or recipients with complicated matching requirements.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:44:14.270.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJoseph Hawksworth
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-22 02:03:47ID requested
12018-04-26 04:49:11announced
22018-05-01 02:58:43announcedUpdated publication reference for PubMed record(s): 29700043.
32024-10-22 04:44:22announced2024-10-22: Updated project metadata.
Publication List
10.15252/emmm.201708454;
Hawksworth J, Satchwell TJ, Meinders M, Daniels DE, Regan F, Thornton NM, Wilson MC, Dobbe JG, Streekstra GJ, Trakarnsanga K, Heesom KJ, Anstee DJ, Frayne J, Toye AM, Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing. EMBO Mol Med, 10(6):(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Erythroid, Blood, CRISPR, BEL-A. Transfusion, Universal Donor
Contact List
Ashley Mark Toye
contact affiliationDepartment of Biochemistry, Biomedical Sciences Building, University of Bristol, Bristol, United Kingdom
contact emailash.m.toye@bristol.ac.uk
lab head
Joseph Hawksworth
contact affiliationUniversity of Bristol
contact emailjoseph.hawksworth@bristol.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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