PXD009291

PXD009291 is an original dataset announced via ProteomeXchange.

Title	Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing
Description	Regular blood transfusion is the cornerstone of care for patients with red blood cell (RBC) disorders such as thalassemia or sickle cell disease. With repeated transfusion, alloimmunisation often occurs due to incompatibility at the level of minor blood group antigens. We use CRISPR-mediated genome editing of an immortalised human erythroblast cell line (BEL-A) to generate multiple enucleation competent cell lines deficient in individual blood groups. Edits are combined to generate a single cell line deficient in multiple antigens responsible for the most common transfusion incompatibilities: ABO (Bombay phenotype), Rh (Rhnull), Kell (K0), Duffy (Duffynull), GPB (S- s- U-). These cells can be differentiated to generate deformable reticulocytes, illustrating the capacity for coexistence of multiple rare blood group antigen null phenotypes. This study provides the first proof-of-principle demonstration of combinatorial CRISPR-mediated blood group gene editing to generate customisable or multi-compatible RBCs for diagnostic reagents or recipients with complicated matching requirements.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:14.270.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joseph Hawksworth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-22 02:03:47	ID requested
1	2018-04-26 04:49:11	announced
2	2018-05-01 02:58:43	announced	Updated publication reference for PubMed record(s): 29700043.
⏵ 3	2024-10-22 04:44:22	announced	2024-10-22: Updated project metadata.

10.15252/emmm.201708454;

Hawksworth J, Satchwell TJ, Meinders M, Daniels DE, Regan F, Thornton NM, Wilson MC, Dobbe JG, Streekstra GJ, Trakarnsanga K, Heesom KJ, Anstee DJ, Frayne J, Toye AM, Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing. EMBO Mol Med, 10(6):(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Erythroid, Blood, CRISPR, BEL-A. Transfusion, Universal Donor

Ashley Mark Toye
contact affiliation	Department of Biochemistry, Biomedical Sciences Building, University of Bristol, Bristol, United Kingdom
contact email	ash.m.toye@bristol.ac.uk
lab head
Joseph Hawksworth
contact affiliation	University of Bristol
contact email	joseph.hawksworth@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD009291

PRIDE project URI

• PRIDE
  1. PXD009291
     1. Label: PRIDE project
     2. Name: Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing