PXD009289

PXD009289 is an original dataset announced via ProteomeXchange.

Title	Helpful dirt: Why diphtheria toxoid vaccines may also protect against non-toxigenic Corynebacterium diphtheriae strains
Description	Diphtheria toxoid vaccines are among the oldest and safest vaccines known. The basic principle of production is the inactivation of purified diphtheria toxin by formaldehyde cross-linking, which converts the potentially fatal toxin in a completely harmless protein aggregate, which is still immunogenic. Since in addition to diphtheria toxin also other proteins might be secreted by Corynebacterium diphtheriae, we assumed that diphtheria toxoid might not be the only component present in the vaccine. Therefore, we established a protocol to reverse formaldehyde cross-linking and carried out mass spectrometric analyses. Different secreted, membrane-associated and cytoplasmic proteins of C. diphtheriae were detected in several vaccine preparations from across the world. Western blot analyses indicated that these are immunogenic and may therefore support protection against C. diphtheriae. Furthermore, we could show that the vaccines also induce antibodies directed against diphtheria toxin secreted by Corynebacterium ulcerans, an emerging pathogen evoking diphtheria-like illness and skin infections.
HostingRepository	PRIDE
AnnounceDate	2019-04-17
AnnouncementXML	Submission_2019-04-17_11:06:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Möller
SpeciesList	scientific name: Corynebacterium diphtheriae NCTC 13129; NCBI TaxID: 257309; scientific name: Clostridium tetani E88; NCBI TaxID: 212717;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-22 01:02:17	ID requested
⏵ 1	2019-04-17 11:06:36	announced

M, ö, ller J, Kraner ME, Burkovski A, Toxoid Vaccines. Proteomes, 7(2):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: corynebacteria, Corynebacterium ulcerans, exoproteome, mass spectrometry, proteomics, secretome, vaccination

Andreas Burkovski
contact affiliation	Department Biologie Professur für Mikrobiologie
contact email	Andreas.burkovski@fau.de
lab head
Jens Möller
contact affiliation	FAU Erlangen
contact email	Jens.moeller@fau.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD009289

PRIDE project URI

• PRIDE
  1. PXD009289
     1. Label: PRIDE project
     2. Name: Helpful dirt: Why diphtheria toxoid vaccines may also protect against non-toxigenic Corynebacterium diphtheriae strains