PXD009280

PXD009280 is an original dataset announced via ProteomeXchange.

Title	Comparative secretome analysis of primary murine white and brown adipocytes
Description	The adipose organ, including white and brown adipose tissues, is an important player in systemic energy homeostasis, storing excess energy in form of lipids while releasing energy upon various energy demands. Recent studies have demonstrated that white and brown adipocytes also function as endocrine cells and regulate systemic metabolism by secreting factors that act locally and systemically. However, a comparative proteomic analysis of secreted factors from white and brown adipocytes and their responsiveness to adrenergic stimulation has not been reported yet. Therefore, we studied and compared the secretome of white and brown adipocytes, with and without norepinephrine (NE) stimulation. Our results reveal that in the absence of NE, carbohydrate metabolism-regulating proteins are preferably secreted from white adipocytes, while brown adipocytes predominantly secrete integrin signaling proteins. Upon NE stimulation, white adipocytes secrete more proteins involved in lipid metabolism, while brown adipocytes secrete more proteins with specific anti-inflammatory properties. In conclusion, our study provides a comprehensive catalogue of novel adipokine candidates secreted from white and brown adipocytes with many of them responsive to NE.
HostingRepository	PRIDE
AnnounceDate	2018-09-04
AnnouncementXML	Submission_2018-09-04_08:08:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophia Foehr
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	isotope labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-21 06:01:00	ID requested
⏵ 1	2018-09-04 08:08:30	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Secretome, white adipocytes, brown adipocytes, murine SVF cells, adrenergic stimulation

Jeroen Krijgsveld
contact affiliation	German Cancer Research Center DKFZ
contact email	j.krijgsveld@dkfz.de
lab head
Sophia Foehr
contact affiliation	DKFZ
contact email	sophia.foehr@dkfz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009280

PRIDE project URI

• PRIDE
  1. PXD009280
     1. Label: PRIDE project
     2. Name: Comparative secretome analysis of primary murine white and brown adipocytes