PXD009278

PXD009278 is an original dataset announced via ProteomeXchange.

Title	Development of MS-based methods for identification and quantification of proteins altered during early pregnancy in dogs
Description	Unlike the situation in humans, no specific biomarker has been related to early pregnancy in dogs. Such a biomarker would be beneficial for animal welfare reasons, e.g. in cases when the bitch has been mismated, and would also be of value for the breeder, to enable better planning for an expected litter. In the present study, a shotgun proteomic analysis based on nano-liquid chromatography coupled to high resolution MS was performed to identify differentially expressed proteins during canine pregnancy, and thereafter, a targeted PRM method was developed and applied to absolutely quantify the concentrations of a selection of them.
HostingRepository	PRIDE
AnnounceDate	2019-08-20
AnnouncementXML	Submission_2019-08-20_06:50:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Valdés
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-21 04:55:27	ID requested
⏵ 1	2019-08-20 06:50:53	announced

Vald, é, s A, Holst BS, Lindersson S, Ramstr, ö, m M, Development of MS-based methods for identification and quantification of proteins altered during early pregnancy in dogs. J Proteomics, 192():223-232(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Dog proteomics, early pregnancy diagnosis, PRM, shotgun proteomics

Bodil Ström Holst
contact affiliation	Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
contact email	Bodil.Strom-Holst@slu.se
lab head
Alberto Valdés
contact affiliation	Department of Chemistry-BMC, Analytical Chemistry, Uppsala University, Uppsala, Sweden
contact email	a.valdes@csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD009278

PRIDE project URI

• PRIDE
  1. PXD009278
     1. Label: PRIDE project
     2. Name: Development of MS-based methods for identification and quantification of proteins altered during early pregnancy in dogs