PXD009277

PXD009277 is an original dataset announced via ProteomeXchange.

Title	Development of a parallel reaction monitoring-MS method to quantify IGF proteins in dogs: application to a canine case study of NICTH syndrome
Description	Non–islet-cell tumor hypoglycemia (NICTH) is a rare paraneoplastic phenomenon seen in both dogs and humans. NICTH syndrome is derived from incompletely processed forms of insulin-like growth factor–II (IGF–II) by tumors, commonly named as big IGF–II. In the present study, a previously developed targeted PRM MS-based method for cats have been optimized and applied to simultaneously quantify the levels of IGF–I, IGF–II, and IGFBP–3, and for the first time, the levels of big IGF–II in dogs. This method allows the absolute quantification of these proteins using a mixture of QPrEST™ proteins previously designed for humans, reducing the variations due to the methodology, and the amount of serum needed for the analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:14.512.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Valdés
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-21 03:22:05	ID requested
1	2018-11-04 13:31:30	announced
⏵ 2	2024-10-22 04:44:22	announced	2024-10-22: Updated project metadata.

Vald, é, s A, Lewitt M, Wiss E, Ramstr, ö, m M, Strage EM, Development of a Parallel Reaction Monitoring-MS Method To Quantify IGF Proteins in Dogs and a Case of Nonislet Cell Tumor Hypoglycemia. J Proteome Res, 18(1):18-29(2019) [pubmed]

10.1021/acs.jproteome.8b00259;

curator keyword: Biological, Biomedical

submitter keyword: Dog proteomics, NICTH, PRM, IGF

Emma M. Strage
contact affiliation	Department of Clinical Sciences, and Clinical Pathology Laboratory, University Animal Hospital, Swedish University of Agricultural Sciences, Uppsala, Sweden.
contact email	Emma.Strage@uds.slu.se
lab head
Alberto Valdés
contact affiliation	Department of Chemistry-BMC, Analytical Chemistry, Uppsala University, Uppsala, Sweden
contact email	a.valdes@csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009277

PRIDE project URI

• PRIDE
  1. PXD009277
     1. Label: PRIDE project
     2. Name: Development of a parallel reaction monitoring-MS method to quantify IGF proteins in dogs: application to a canine case study of NICTH syndrome