PXD009276

PXD009276 is an original dataset announced via ProteomeXchange.

Title	RPL12 phosphorylation regulates translation during mitosis - pSILAC B cell -
Description	Emerging evidence indicates that heterogeneity in ribosome composition can give rise to specialized functions. Until now, research mainly focused on differences in core ribosomal proteins and associated factors. The impact of posttranslational modifications has not yet been studied systematically. Analyzing ribosome heterogeneity is challenging since individual proteins can be part of different subcomplexes (40S, 60S, 80S and polysomes). Here, we develop polysome proteome profiling (3P) to obtain unbiased proteomic maps across ribosomal subcomplexes. 3P combines extensive fractionation by sucrose gradient centrifugation with quantitative mass spectrometry. The high resolution of the profiles allows us to assign proteins to specific subcomplexes. Phosphoproteomics on 3P fractions reveals that phosphorylation of serine 38 in RPL12 -- a known mitotic CDK1 substrate -- is strongly depleted in polysomes. Follow-up experiments confirm that RPL12 phosphorylation regulates translation of specific subsets of mRNAs during mitosis. Together, our results show that posttranslational modification of ribosomal proteins can regulate translation.
HostingRepository	PRIDE
AnnounceDate	2018-08-31
AnnouncementXML	Submission_2018-10-19_03:43:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Koshi Imami
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-21 02:42:15	ID requested
1	2018-08-31 05:41:39	announced
⏵ 2	2018-10-19 03:43:37	announced	Updated publication reference for PubMed record(s): 30220558.

Imami K, Milek M, Bogdanow B, Yasuda T, Kastelic N, Zauber H, Ishihama Y, Landthaler M, Selbach M, Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis. Mol Cell, 72(1):84-98.e9(2018) [pubmed]

curator keyword: Biological

submitter keyword: Ribosome, LC-MS

Matthias Selbach
contact affiliation	MDC
contact email	matthias.selbach@mdc-berlin.de
lab head
Koshi Imami
contact affiliation	MDC
contact email	liqerume2000@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009276

PRIDE project URI

• PRIDE
  1. PXD009276
     1. Label: PRIDE project
     2. Name: RPL12 phosphorylation regulates translation during mitosis - pSILAC B cell -