PXD009263
PXD009263 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | (3S)-Lysyl hydroxylation of TRAFAC GTPases is catalyzed by the 1 Jumonji-C oxygenase JMJD7 |
| Description | Biochemical, structural, and cellular studies reveal Jumonji-C (JmjC) domain-containing (JMJD7) as a 2-oxoglutarate (2OG)-dependent oxygenase catalyzing a previously unreported type of post translational modification, (3S)-lysyl hydroxylation. Crystallographic analyses reveal JMJD7 as more closely related to the JmjC hydroxylases rather than the JmjC demethylases. Biophysical and mutation studies show that JMJD7 has a unique dimerization mode, with interactions between monomers involving both N- and C-terminal regions and disulfide bond formation. A proteomic approach identifies two related members of the Translation Factor (TRAFAC) family of GTPases, Developmentally Regulated GTP Binding Proteins 1 and 2 (DRG1/2), as activity-dependent JMJD7 interactors. Mass spectrometric analyses demonstrate that JMJD7 catalyzes Fe(II)- and 2OG-dependent hydroxylation of a highly-conserved lysine residue in DRG1/2; amino acid analyses reveal JMJD7 catalyzes (3S)-lysyl hydroxylation. The functional assignment of JMJD7 will enable future studies to define the role of DRG hydroxylation in cell growth and disease. MSQ506 - hydroxylation of DRG1/2 detected after assay with Jmjd7 wt, H178A mutant and empty vector (analysed with Q-Exacrtive classic, Progenesis QI and Mascot) MSQ784 - as above (analysed Q-Exactive classic, Progenesis QI and PEAKS) MSQ849 - Hydroxylation of endogenous DRG (analysed with Q-Exactive HF and PEAKS) MSQ884 - as above (analysed with Orebitrap Fusion Lumos and PEAKS) |
| HostingRepository | MassIVE |
| AnnounceDate | 2018-05-21 |
| AnnouncementXML | Submission_2018-05-21_03:30:52.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Roman Fischer |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Fusion ETD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-03-20 03:43:29 | ID requested | |
| ⏵ 1 | 2018-05-21 03:30:52 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: JMJD7, Hydroxylation |
Contact List
| Mathew Coleman | |
|---|---|
| contact affiliation | University of Birmingham |
| contact email | M.Coleman@bham.ac.uk |
| lab head | |
| Roman Fischer | |
| contact affiliation | University of Oxford |
| contact email | roman.fischer@ndm.ox.ac.uk |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082185 |
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