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PXD009257

PXD009257 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAsian citrus psyllid hemolymph LC-MS/MS
DescriptionCitrus greening disease, also known as Huanglongbing (HLB), is the most serious disease of citrus plants. It is associated with the Gram-negative bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), which is transmitted between host plants by the hemipteran insect vector Diaphorina citri in a circulative, propagative manner involving specific interactions with various insect tissues, including the hemolymph, fluid that occupies the body cavity akin to insect blood. High resolution quantitative mass spectrometry was performed to investigate the effect of CLas exposure on D. citri hemolymph at the proteome level. In contrast to the broad proteome effects on hundreds of proteins and a diverse array of metabolic pathways previously reported in gut and whole insect proteome analyses, the effect of CLas on the hemolymph was observed to be highly specific, restricted to key immunity and metabolism pathways, and lower in magnitude than that previously observed in the whole insect body and gut. Vitellogenins were abundantly expressed and CLas-responsive. Gene-specific RNA expression analysis suggests that these proteins are expressed in both male and female insects, and may have roles outside of reproductive vitellogenesis. Proteins for fatty acid synthesis were found to be up-regulated, along with metabolic proteins associated with energy production, supported at the organismal level by the previously published observation that D. citri individuals experience a higher level of hunger when reared on CLas-infected plants. Prediction of post-translational modifications identified hemolymph proteins with phosphorylation and acetylation upon CLas exposure. Proteins derived from the three most prominent bacterial endosymbionts of the psyllid were also detected in the hemolymph, and several of these have predicted secretion signals. A DNAK protein, the bacterial HSP70, detected in the hemolymph expressed from Wolbachia pipientis was predicted to encode a eukaryotic nuclear localization signal. Taken together, these data show specific changes to immunity and metabolism in D. citri hemolymph involving host and endosymbiont proteins. These data provide a novel context for proteomic changes seen in other D. citri tissues in response to CLas and align with organismal data on the effects of CLas on D. citri metabolism and reproduction.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:32:58.874.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD009257
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAngela Kruse
SpeciesList scientific name: Diaphorina citri (Asian citrus psyllid); NCBI TaxID: 121845;
ModificationListMethylthio; Oxidation
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-19 08:57:26ID requested
12018-08-16 08:27:51announced
22024-10-22 04:33:03announced2024-10-22: Updated project metadata.
Publication List
Kruse A, Ramsey JS, Johnson R, Hall DG, MacCoss MJ, Heck M, Candidatus Liberibacter asiaticus Minimally Alters Expression of Immunity and Metabolism Proteins in Hemolymph of Diaphorina citri, the Insect Vector of Huanglongbing. J Proteome Res, 17(9):2995-3011(2018) [pubmed]
10.6019/PXD009257;
10.1021/acs.jproteome.8b00183;
Keyword List
curator keyword: Biological
submitter keyword: hemolymph, vitellogenin, Diaphorina citri, citrus,Psyllid, Candidatus Liberibacter asiaticus, LC-MS/MS
Contact List
Michelle Heck
contact affiliationUSDA-ARS, Boyce Thompson Institute, Cornell University
contact emailmlc68@cornell.edu
lab head
Angela Kruse
contact affiliationVanderbilt University
contact emailark253@cornell.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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