PXD009257

PXD009257 is an original dataset announced via ProteomeXchange.

Title	Asian citrus psyllid hemolymph LC-MS/MS
Description	Citrus greening disease, also known as Huanglongbing (HLB), is the most serious disease of citrus plants. It is associated with the Gram-negative bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), which is transmitted between host plants by the hemipteran insect vector Diaphorina citri in a circulative, propagative manner involving specific interactions with various insect tissues, including the hemolymph, fluid that occupies the body cavity akin to insect blood. High resolution quantitative mass spectrometry was performed to investigate the effect of CLas exposure on D. citri hemolymph at the proteome level. In contrast to the broad proteome effects on hundreds of proteins and a diverse array of metabolic pathways previously reported in gut and whole insect proteome analyses, the effect of CLas on the hemolymph was observed to be highly specific, restricted to key immunity and metabolism pathways, and lower in magnitude than that previously observed in the whole insect body and gut. Vitellogenins were abundantly expressed and CLas-responsive. Gene-specific RNA expression analysis suggests that these proteins are expressed in both male and female insects, and may have roles outside of reproductive vitellogenesis. Proteins for fatty acid synthesis were found to be up-regulated, along with metabolic proteins associated with energy production, supported at the organismal level by the previously published observation that D. citri individuals experience a higher level of hunger when reared on CLas-infected plants. Prediction of post-translational modifications identified hemolymph proteins with phosphorylation and acetylation upon CLas exposure. Proteins derived from the three most prominent bacterial endosymbionts of the psyllid were also detected in the hemolymph, and several of these have predicted secretion signals. A DNAK protein, the bacterial HSP70, detected in the hemolymph expressed from Wolbachia pipientis was predicted to encode a eukaryotic nuclear localization signal. Taken together, these data show specific changes to immunity and metabolism in D. citri hemolymph involving host and endosymbiont proteins. These data provide a novel context for proteomic changes seen in other D. citri tissues in response to CLas and align with organismal data on the effects of CLas on D. citri metabolism and reproduction.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:58.874.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009257
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Angela Kruse
SpeciesList	scientific name: Diaphorina citri (Asian citrus psyllid); NCBI TaxID: 121845;
ModificationList	Methylthio; Oxidation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-19 08:57:26	ID requested
1	2018-08-16 08:27:51	announced
⏵ 2	2024-10-22 04:33:03	announced	2024-10-22: Updated project metadata.

Kruse A, Ramsey JS, Johnson R, Hall DG, MacCoss MJ, Heck M, Candidatus Liberibacter asiaticus Minimally Alters Expression of Immunity and Metabolism Proteins in Hemolymph of Diaphorina citri, the Insect Vector of Huanglongbing. J Proteome Res, 17(9):2995-3011(2018) [pubmed]

10.6019/PXD009257;

10.1021/acs.jproteome.8b00183;

curator keyword: Biological

submitter keyword: hemolymph, vitellogenin, Diaphorina citri, citrus,Psyllid, Candidatus Liberibacter asiaticus, LC-MS/MS

Michelle Heck
contact affiliation	USDA-ARS, Boyce Thompson Institute, Cornell University
contact email	mlc68@cornell.edu
lab head
Angela Kruse
contact affiliation	Vanderbilt University
contact email	ark253@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009257
     1. Label: PRIDE project
     2. Name: Asian citrus psyllid hemolymph LC-MS/MS