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PXD009243

PXD009243 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTwenty-four hour rhythms in the absence of the clock gene Bmal1
DescriptionCircadian (~24 hour) clocks exist in almost all types of living organism and play a fundamental role in regulating daily physiological and behavioural processes. The transcription factor BMAL1 (ARNTL) is thought to be one of the principal drivers of the molecular clock in mammals since its deletion abolishes 24-hour activity patterning, an important physiological output of the clockwork. However, whether or not Bmal1-/- mice can nevertheless display molecular 24-hour rhythms is unknown. Here, we determined whether Bmal1 function is necessary for daily molecular oscillations in two tissues – skin fibroblasts and liver. Unexpectedly, both tissues exhibited robust 24-hour oscillations over 2-3 days in the absence of any exogenous synchronizers such as daily light or temperature cycles. This demonstrates a competent 24-hour molecular pacemaker in Bmal1 knockouts. Indeed, molecular oscillations were pervasive throughout the transcriptome, proteome and phosphoproteome of Bmal1-/- mice. In particular, several proteins exhibited rhythmic phosphorylation in both Bmal1-proficient and -deficient cells, highlighting an unanticipated role for post-translational regulators in 24-hour rhythms in the absence of any known clock mechanisms.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:59:37.039.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSandipan Ray
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-16 09:27:02ID requested
12020-02-17 07:21:39announced
22024-10-22 04:59:37announced2024-10-22: Updated project metadata.
Publication List
10.1126/science.aaw7365;
Ray S, Valekunja UK, Stangherlin A, Howell SA, Snijders AP, Damodaran G, Reddy AB, . Science, 367(6479):800-806(2020) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Bmal1
Circadian rhythms
Kinase network
Non-transcriptional oscillators
Rhythmic proteome and phosphoproteome
Contact List
Akhilesh B. Reddy
contact affiliationThe Francis Crick Institute, 1 Midland Road, London NW1 1AT, United Kingdom
contact emailareddy@cantab.net
lab head
Sandipan Ray
contact affiliationUniversity of Pennsylvania
contact emailsandipan.ray@cantab.net
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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