PXD009241

PXD009241 is an original dataset announced via ProteomeXchange.

Title	Identification of potential biomarkers of head and neck squamous cell carcinoma using iTRAQ based quantitative proteomic approach
Description	Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers in India. Despite improvements in treatment strategy, the survival rates of HNSCC patients remains poor. Thus, it is necessary to identify biomarkers that can be used for early detection of disease. In this study, we employed iTRAQ-based quantitative mass spectrometry analysis to identify dysregulated proteins from a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. We identified 2,468 proteins, of which 496 proteins were found to be dysregulated in at least two HNSCC cell lines compared to immortalized normal oral keratinocytes. We detected increased expression of replication protein A1 (RPA1) and heat shock protein family H (Hsp110) member 1 (HSPH1), in HNSCC cell lines compared to control. The differentially expressed proteins were further validated using parallel reaction monitoring (PRM) and western blot analysis in HNSCC cell lines. Immunohistochemistry-based validation using HNSCC tissue microarrays revealed overexpression of RPA1 and HSPH1 in 15.7% and 32.2% of the tested cases, respectively. Our study illustrates quantitative proteomics as a robust approach for identification of potential HNSCC biomarkers.
HostingRepository	PRIDE
AnnounceDate	2018-09-19
AnnouncementXML	Submission_2018-09-19_03:48:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aditi Chatterjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-16 08:04:33	ID requested
⏵ 1	2018-09-19 03:48:34	announced

Babu N, Mohan S, Nanjappa V, Chavan S, Advani J, Khan AA, Renuse S, Radhakrishnan A, Prasad TSK, Kumar RV, Ray JG, Biswas M, Thiyagarajan S, Califano JA, Sidransky D, Gowda H, Chatterjee A, Identification of potential biomarkers of head and neck squamous cell carcinoma using iTRAQ based quantitative proteomic approach. Data Brief, 19():1124-1130(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: HNSCC, iTRAQ, Parallel Reaction Monitoring, Mass Spectrometry, OKF6/TERT

Aditi Chatterjee
contact affiliation	Institute of Bioinformatics 7th floor, Discoverer building, International Tech Park, Bangalore 560 066
contact email	aditixchatterjee@gmail.com
lab head
Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India
contact email	aditixchatterjee@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009241

PRIDE project URI

• PRIDE
  1. PXD009241
     1. Label: PRIDE project
     2. Name: Identification of potential biomarkers of head and neck squamous cell carcinoma using iTRAQ based quantitative proteomic approach