PXD009234

PXD009234 is an original dataset announced via ProteomeXchange.

Title	Global phosphoproteomic analysis identifies SRMS-regulated signaling intermediates
Description	The non-receptor tyrosine kinase, SRMS (Src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristoylation sites) is a member of the BRK family kinases (BFKs) which represents an evolutionarily conserved relative of the Src family kinases (SFKs). Tyrosine kinases are known to regulate a number of cellular processes and pathways via phosphorylating substrate proteins directly and/or by partaking in signaling cross-talks leading to the indirect modulation of various signaling intermediates. In a previous study, we profiled the tyrosine-phosphoproteome of SRMS and identified multiple candidate substrates of the kinase. In order to uncover the broader SRMS-regulated phosphoproteome and identify the SRMS-regulated indirect signaling intermediates, we performed global phosphoproteomics analysis on cells expressing wild-type SRMS. Our analyses identified 60 hyperphosphorylated (phosphoserine/phosphothreonine) proteins mapped from 140 hyperphosphorylated peptides. Bioinfomatics analyses identified a number of significantly enriched biological and cellular processes among which DNA repair pathways were found to be upregulated while apoptotic pathways were found to be downregulated. Analyses of motifs derived from the upregulated phosphosites identified Casein kinase 2 alpha (CK2) as one of the major potential kinases contributing to the SRMS-dependent indirect regulation of signaling intermediates. Overall, our phosphoproteomics analyses identified serine/threonine phosphorylation dynamics as important secondary events of the SRMS-regulated phosphoproteome with implications in the regulation of cellular and biological processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:43.762.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raghuveera Goel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-16 07:29:02	ID requested
1	2018-10-19 11:43:56	announced
⏵ 2	2024-10-22 04:43:52	announced	2024-10-22: Updated project metadata.

10.1186/s12953-018-0143-7;

Goel RK, Meyer M, Paczkowska M, Reimand J, Vizeacoumar F, Vizeacoumar F, Lam TT, Lukong KE, Global phosphoproteomic analysis identifies SRMS-regulated secondary signaling intermediates. Proteome Sci, 16():16(2018) [pubmed]

curator keyword: Biological

submitter keyword: FRK,SRMS, BRK, mass spectrometry, PTK5, PTK6, PTK70, Src, phosphoproteomics, non-receptor tyrosine kinase

Kiven Erique Lukong
contact affiliation	Department of Biochemistry, College of Medicine, University of Saskatchewan
contact email	kel232@mail.usask.ca
lab head
Raghuveera Goel
contact affiliation	University of Saskatchewan
contact email	raghu.goel@usask.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009234
     1. Label: PRIDE project
     2. Name: Global phosphoproteomic analysis identifies SRMS-regulated signaling intermediates