PXD009232

PXD009232 is an original dataset announced via ProteomeXchange.

Title	PGC-1a isoform-specific nuclear interactome
Description	PGC-1α (Peroxisome proliferator-activated receptor gamma coactivator-1alpha) coactivators regulate adaptive gene expression in response to challenges such as cold exposure, fasting, or physical exercise to balance energy supply and demand. Transcription of a single PGC-1α gene produces different isoforms (e.g. PGC-1α1 to α4) with different biological functions. We aimed to characterize the nuclear interactome for each PGC-1α variant, in particular the transcription factors they bind to regulate gene expression. This was done by generating GST-fusions of all PGC-1a variants, expressed in an insect cell system. These were used to capture associated protein complexes from HeLa nuclear extracts.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_09:47:40.979.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lars Ketscher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-16 07:22:05	ID requested
⏵ 1	2022-03-01 09:47:41	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Human, Hela, Interactome

Jorge L Ruas
contact affiliation	Associate Professor of Molecular Physiology at Karolinska Institutet Molecular & Cellular Exercise Physiology Dept. of Physiology and Pharmacology Karolinska Institutet Stockholm, Sweden
contact email	jorge.ruas@ki.se
lab head
Lars Ketscher
contact affiliation	KI Stockholm
contact email	lars.ketscher@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD009232

PRIDE project URI

• PRIDE
  1. PXD009232
     1. Label: PRIDE project
     2. Name: PGC-1a isoform-specific nuclear interactome