PXD009225

PXD009225 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome of Francicella Tularensis
Description	The pathogenic bacterium Francisella tularensis possesses a non-canonical T6SS encoded on the Francisella pathogenicity island (FPI) that is essential for efficient phagosomal escape and access to the cytosolic compartment of infected host cells. Using a global and site-specific phosphoproteomic analysis of F. novicida, we identified a phosphorylated form of IglB, which constitutes with iglA the outer sheath of the T6SS. We show here that substitution of the unique phosphorylated tyrosine (Y139) of IglB to alanine or to the non-phosphorylatable analogue phenylalanine prevents the formation of the sheath-like structures and impairs normal bacterial phagosomal escape. We propose that IglB phosphorylation is involved in the dynamics of assembly-disassembly of the sheath.
HostingRepository	PRIDE
AnnounceDate	2021-03-29
AnnouncementXML	Submission_2021-03-29_09:49:35.982.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Francisella tularensis subsp. novicida (strain U112); NCBI TaxID: 401614;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-15 09:31:58	ID requested
⏵ 1	2021-03-29 09:49:36	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Francicella Tularensis, phospho

GUerrera I C
contact affiliation	Proteomics Laboratory Structure Federative Recherche Necker, INserm US24Paris , France
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD009225

PRIDE project URI

• PRIDE
  1. PXD009225
     1. Label: PRIDE project
     2. Name: Phosphoproteome of Francicella Tularensis