PXD009220

PXD009220 is an original dataset announced via ProteomeXchange.

Title	Integrative proteomic and phosphoproteomic profiling of testis from Wip1-knockout mice: insights into mechanisms of reduced fertility
Description	Spermatogenesis plays an important role in the mammalian testis, involving in the complex processes of mitosis, meiosis, and spermiogenesis. Spermatogenesis may also be disrupted in the absence of the immunological and ‘fence’ functions of the BTB, resulting in male subfertility or infertility. Mice lacking wild-type p53-induced phosphatase 1 (Wip1) display male reproductive organ defects, but the molecular mechanisms underlying these abnormalities remain unclear. We explored the function of Wip1 in spermatogenesis and fertility by examining differences in the expressed testis proteome and phosphoproteome between Wip1-deficient and wild-type mice using a proteomics approach. Among a total of 6872 proteins and 4280 phosphorylation sites on 1614 proteins identified in our analysis, 58 proteins and 159 phosphorylation sites on 141 proteins were differentially regulated between these two groups of mice. These results suggested that proinflammatory cytokines may impair the blood–testis barrier dynamics by decreasing the expression of junction-associated proteins, which effect could be partially responsible for the subfertility and spermatogenesis defects in Wip1-knockout mice.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:22.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yinghui wei
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-15 07:38:06	ID requested
1	2018-11-02 02:48:38	announced
⏵ 2	2024-10-22 04:45:31	announced	2024-10-22: Updated project metadata.

Wei Y, Gao Q, Niu P, Xu K, Qiu Y, Hu Y, Liu S, Zhang X, Yu M, Liu Z, Wang B, Mu Y, Li K, Integrative Proteomic and Phosphoproteomic Profiling of Testis from Wip1 Phosphatase-Knockout Mice: Insights into Mechanisms of Reduced Fertility. Mol Cell Proteomics, 18(2):216-230(2019) [pubmed]

10.1074/mcp.ra117.000479;

curator keyword: Biological

submitter keyword: Fertility,Proteomic, Phosphoproteomic, Knockout mice, Testis, Wip1

Kui Li
contact affiliation	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
contact email	likui@caas.cn
lab head
yinghui wei
contact affiliation	Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
contact email	yinghuiwei1019@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009220

PRIDE project URI

• PRIDE
  1. PXD009220
     1. Label: PRIDE project
     2. Name: Integrative proteomic and phosphoproteomic profiling of testis from Wip1-knockout mice: insights into mechanisms of reduced fertility