PXD009220 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrative proteomic and phosphoproteomic profiling of testis from Wip1-knockout mice: insights into mechanisms of reduced fertility |
Description | Spermatogenesis plays an important role in the mammalian testis, involving in the complex processes of mitosis, meiosis, and spermiogenesis. Spermatogenesis may also be disrupted in the absence of the immunological and ‘fence’ functions of the BTB, resulting in male subfertility or infertility. Mice lacking wild-type p53-induced phosphatase 1 (Wip1) display male reproductive organ defects, but the molecular mechanisms underlying these abnormalities remain unclear. We explored the function of Wip1 in spermatogenesis and fertility by examining differences in the expressed testis proteome and phosphoproteome between Wip1-deficient and wild-type mice using a proteomics approach. Among a total of 6872 proteins and 4280 phosphorylation sites on 1614 proteins identified in our analysis, 58 proteins and 159 phosphorylation sites on 141 proteins were differentially regulated between these two groups of mice. These results suggested that proinflammatory cytokines may impair the blood–testis barrier dynamics by decreasing the expression of junction-associated proteins, which effect could be partially responsible for the subfertility and spermatogenesis defects in Wip1-knockout mice. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:45:22.940.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | yinghui wei |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-03-15 07:38:06 | ID requested | |
1 | 2018-11-02 02:48:38 | announced | |
⏵ 2 | 2024-10-22 04:45:31 | announced | 2024-10-22: Updated project metadata. |
Publication List
Wei Y, Gao Q, Niu P, Xu K, Qiu Y, Hu Y, Liu S, Zhang X, Yu M, Liu Z, Wang B, Mu Y, Li K, Integrative Proteomic and Phosphoproteomic Profiling of Testis from Wip1 Phosphatase-Knockout Mice: Insights into Mechanisms of Reduced Fertility. Mol Cell Proteomics, 18(2):216-230(2019) [pubmed] |
10.1074/mcp.ra117.000479; |
Keyword List
curator keyword: Biological |
submitter keyword: Fertility,Proteomic, Phosphoproteomic, Knockout mice, Testis, Wip1 |
Contact List
Kui Li |
contact affiliation | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
contact email | likui@caas.cn |
lab head | |
yinghui wei |
contact affiliation | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
contact email | yinghuiwei1019@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009220
- Label: PRIDE project
- Name: Integrative proteomic and phosphoproteomic profiling of testis from Wip1-knockout mice: insights into mechanisms of reduced fertility