PXD009214

PXD009214 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS shotgun proteome analysis of Saccharomyces cerevisiae lysates
Description	N-terminal acetylation is a highly abundant protein modification in eukaryotes which impacts a wide range of cellular processes and protein properties. This modification was proven to be essential for development and viability of multiple eukaryotes. Still the molecular mechanisms at play and the protein-specific consequences of N-terminal acetylation have only been elucidated for a limited number of substrates. In this label-free shotgun proteomics study we aimed to look at the changes in overall protein abundance linked to N-terminal acetylation levels. BY4742 yeast cell pellets were obtained at 6 different metabolic conditions: yeast cultures grown for 6 h, 3 days, 8 days, 9 days + 0% glucose, 9 days + 2% glucose, and 9 days + 10% of glucose. Cells were lysed with urea buffer, extracted proteins were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. Proteins were identified and quantified with the MaxQuant software leading to identification of 2,707 proteins and quantification of 1,918 proteins. A two-way ANOVA test was performed to reveal 401 proteins which were significantly regulated between the different metabolic conditions. Gene ontology analysis revealed a strong enrichment for genes involved in oxidation-reduction processes and translation. Yeast cells redirect their cellular metabolism from fermentation to respiration when glucose becomes exhausted from the growth medium. This reprogramming is accompanied by reduced gene expression and protein translation. Thus, these findings are in agreement with known proteome changes in response to prolonged growth.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:39.863.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Delphi Van Haver
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-15 06:33:40	ID requested
1	2018-11-15 13:57:44	announced
⏵ 2	2024-10-22 04:43:47	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra118.000982;

Varland S, Aksnes H, Kryuchkov F, Impens F, Van Haver D, Jonckheere V, Ziegler M, Gevaert K, Van Damme P, Arnesen T, . Mol Cell Proteomics, 17(12):2309-2323(2018) [pubmed]

curator keyword: Biological

submitter keyword: Tandem Mass spectrometry, Saccharomyces cerevisiae,N-terminal Acetylation

Thomas Arnesen
contact affiliation	University of Bergen, N-5020 Bergen, Norway
contact email	Thomas.Arnesen@uib.no
lab head
Delphi Van Haver
contact affiliation	VIB Proteomics Core
contact email	delphi.vanhaver@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009214

PRIDE project URI

• PRIDE
  1. PXD009214
     1. Label: PRIDE project
     2. Name: LC-MS/MS shotgun proteome analysis of Saccharomyces cerevisiae lysates