PXD009214 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS shotgun proteome analysis of Saccharomyces cerevisiae lysates |
Description | N-terminal acetylation is a highly abundant protein modification in eukaryotes which impacts a wide range of cellular processes and protein properties. This modification was proven to be essential for development and viability of multiple eukaryotes. Still the molecular mechanisms at play and the protein-specific consequences of N-terminal acetylation have only been elucidated for a limited number of substrates. In this label-free shotgun proteomics study we aimed to look at the changes in overall protein abundance linked to N-terminal acetylation levels. BY4742 yeast cell pellets were obtained at 6 different metabolic conditions: yeast cultures grown for 6 h, 3 days, 8 days, 9 days + 0% glucose, 9 days + 2% glucose, and 9 days + 10% of glucose. Cells were lysed with urea buffer, extracted proteins were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. Proteins were identified and quantified with the MaxQuant software leading to identification of 2,707 proteins and quantification of 1,918 proteins. A two-way ANOVA test was performed to reveal 401 proteins which were significantly regulated between the different metabolic conditions. Gene ontology analysis revealed a strong enrichment for genes involved in oxidation-reduction processes and translation. Yeast cells redirect their cellular metabolism from fermentation to respiration when glucose becomes exhausted from the growth medium. This reprogramming is accompanied by reduced gene expression and protein translation. Thus, these findings are in agreement with known proteome changes in response to prolonged growth. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:43:39.863.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Delphi Van Haver |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-03-15 06:33:40 | ID requested | |
1 | 2018-11-15 13:57:44 | announced | |
⏵ 2 | 2024-10-22 04:43:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra118.000982; |
Varland S, Aksnes H, Kryuchkov F, Impens F, Van Haver D, Jonckheere V, Ziegler M, Gevaert K, Van Damme P, Arnesen T, . Mol Cell Proteomics, 17(12):2309-2323(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Tandem Mass spectrometry, Saccharomyces cerevisiae,N-terminal Acetylation |
Contact List
Thomas Arnesen |
contact affiliation | University of Bergen, N-5020 Bergen, Norway |
contact email | Thomas.Arnesen@uib.no |
lab head | |
Delphi Van Haver |
contact affiliation | VIB Proteomics Core |
contact email | delphi.vanhaver@vib-ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009214 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009214
- Label: PRIDE project
- Name: LC-MS/MS shotgun proteome analysis of Saccharomyces cerevisiae lysates