⮝ Full datasets listing

PXD009214

PXD009214 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS shotgun proteome analysis of Saccharomyces cerevisiae lysates
DescriptionN-terminal acetylation is a highly abundant protein modification in eukaryotes which impacts a wide range of cellular processes and protein properties. This modification was proven to be essential for development and viability of multiple eukaryotes. Still the molecular mechanisms at play and the protein-specific consequences of N-terminal acetylation have only been elucidated for a limited number of substrates. In this label-free shotgun proteomics study we aimed to look at the changes in overall protein abundance linked to N-terminal acetylation levels. BY4742 yeast cell pellets were obtained at 6 different metabolic conditions: yeast cultures grown for 6 h, 3 days, 8 days, 9 days + 0% glucose, 9 days + 2% glucose, and 9 days + 10% of glucose. Cells were lysed with urea buffer, extracted proteins were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. Proteins were identified and quantified with the MaxQuant software leading to identification of 2,707 proteins and quantification of 1,918 proteins. A two-way ANOVA test was performed to reveal 401 proteins which were significantly regulated between the different metabolic conditions. Gene ontology analysis revealed a strong enrichment for genes involved in oxidation-reduction processes and translation. Yeast cells redirect their cellular metabolism from fermentation to respiration when glucose becomes exhausted from the growth medium. This reprogramming is accompanied by reduced gene expression and protein translation. Thus, these findings are in agreement with known proteome changes in response to prolonged growth.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:43:39.863.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDelphi Van Haver
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-03-15 06:33:40ID requested
12018-11-15 13:57:44announced
22024-10-22 04:43:47announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.ra118.000982;
Varland S, Aksnes H, Kryuchkov F, Impens F, Van Haver D, Jonckheere V, Ziegler M, Gevaert K, Van Damme P, Arnesen T, . Mol Cell Proteomics, 17(12):2309-2323(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Tandem Mass spectrometry, Saccharomyces cerevisiae,N-terminal Acetylation
Contact List
Thomas Arnesen
contact affiliationUniversity of Bergen, N-5020 Bergen, Norway
contact emailThomas.Arnesen@uib.no
lab head
Delphi Van Haver
contact affiliationVIB Proteomics Core
contact emaildelphi.vanhaver@vib-ugent.be
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009214
PRIDE project URI
Repository Record List
[ + ]