PXD009210

PXD009210 is an original dataset announced via ProteomeXchange.

Title	Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP)
Description	Serine hydrolases play diverse roles in regulating host-pathogen interactions in a number of organisms, yet few have been characterized in the human pathogen Staphylococcus aureus. Here, we describe a chemical proteomic screen that identified 10 previously uncharacterized S. aureus serine hydrolases that mostly lack human homologues. We termed these enzymes Fluorophosphonate-binding hydrolases (FphA-J). One hydrolase, FphB, can process short fatty acid esters, exhibits increased activity in response to host cell factors, is located predominantly on the bacterial cell surface in a subset of cells, and is concentrated in the division septum. Genetic disruption of the fphB gene confirms that the enzyme is dispensable for bacterial growth in culture but crucial for establishing infection in distinct sites in vivo. A selective small molecule inhibitor of FphB effectively reduces infectivity in vivo, suggesting that it may be a viable therapeutic target for the treatment or management of Staphylococcus infections.
HostingRepository	PRIDE
AnnounceDate	2018-05-17
AnnouncementXML	Submission_2018-05-23_00:54:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eranthie Weerapana
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-14 10:51:30	ID requested
1	2018-05-17 07:46:17	announced
⏵ 2	2018-05-23 00:54:06	announced	Updated publication reference for PubMed record(s): 29769740.

Lentz CS, Sheldon JR, Crawford LA, Cooper R, Garland M, Amieva MR, Weerapana E, Skaar EP, Bogyo M, Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP). Nat Chem Biol, 14(6):609-617(2018) [pubmed]

curator keyword: Biological

submitter keyword: S. aureus, ABPP, serine hydrolase

Eranthie Weerapana
contact affiliation	Boston College
contact email	eranthie@bc.edu
lab head
Eranthie Weerapana
contact affiliation	Boston College
contact email	eranthie@bc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD009210

PRIDE project URI

• PRIDE
  1. PXD009210
     1. Label: PRIDE project
     2. Name: Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP)